EVALUATION OF OUTER MEMBRANE PROTEIN K (OMPK) IN PROTECTING LITOPENAEUS VANNAMEI AGAINST VIBRIO HARVEYI INFECTION
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Date
2020
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
Outer membrane proteins (OMPs) are present in many prokaryotes and in some
organelles of eukaryotic cells. In Gram-negative bacteria, they are considered as the
important molecules as they play various roles in bacterial adaptation including
pathogenicity of bacterium. As OMPs are present on the outer surface of the bacterial cell
and are the first line of contact between the bacterium and its surroundings, they are
considered as good vaccine candidates. Studies have showed that vaccines consisting of
immunogenic fractions can induce higher protection than inactivated whole-cell bacteria
in fish and other vertebrates. Research has shown OMPs extracted from several bacterial
fish pathogens viz., Edwardsiella ictaluri, E. tarda, Vibrio vulnificus, Aeromonas
salmonicida, and A. hydrophila to be protective antigens in fish.
As no study is currently available for the evaluation of the effect of OmpK on
shrimp, present work aims to study the immunogenic potential of OmpK, and its suitability
as a protective candidate against Vibrio harveyi infection in the shrimp, Litopenaeus
vannamei. In the present study, preliminary investigation has been undertaken into existing
OmpK sequences in GenBank database engaging bioinformatics tools to understand the
suitability of OmpK as a vaccine candidate. Primers were designed for the amplification of
OmpK gene of V. harveyi, and the gene was later cloned and expressed using E. coli SG
cells. Further, the protein was purified through Ni-NTA affinity chromatography after
successful confirmation by SDS-PAGE, and its effectiveness against V. harveyi was
evaluated which showed RPS 75 respectively. Our results suggest that OmpK of V. harveyi
could be used as a potential protective candidate for L. vannamei against V. harveyi
infection.