EVALUATION OF OUTER MEMBRANE PROTEIN K (OMPK) IN PROTECTING LITOPENAEUS VANNAMEI AGAINST VIBRIO HARVEYI INFECTION
| dc.contributor.advisor | M.N.VENUGOPAL | |
| dc.contributor.author | SAPTAMI. R. JOGALEKAR | |
| dc.date.accessioned | 2023-12-18T16:22:25Z | |
| dc.date.available | 2023-12-18T16:22:25Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | Outer membrane proteins (OMPs) are present in many prokaryotes and in some organelles of eukaryotic cells. In Gram-negative bacteria, they are considered as the important molecules as they play various roles in bacterial adaptation including pathogenicity of bacterium. As OMPs are present on the outer surface of the bacterial cell and are the first line of contact between the bacterium and its surroundings, they are considered as good vaccine candidates. Studies have showed that vaccines consisting of immunogenic fractions can induce higher protection than inactivated whole-cell bacteria in fish and other vertebrates. Research has shown OMPs extracted from several bacterial fish pathogens viz., Edwardsiella ictaluri, E. tarda, Vibrio vulnificus, Aeromonas salmonicida, and A. hydrophila to be protective antigens in fish. As no study is currently available for the evaluation of the effect of OmpK on shrimp, present work aims to study the immunogenic potential of OmpK, and its suitability as a protective candidate against Vibrio harveyi infection in the shrimp, Litopenaeus vannamei. In the present study, preliminary investigation has been undertaken into existing OmpK sequences in GenBank database engaging bioinformatics tools to understand the suitability of OmpK as a vaccine candidate. Primers were designed for the amplification of OmpK gene of V. harveyi, and the gene was later cloned and expressed using E. coli SG cells. Further, the protein was purified through Ni-NTA affinity chromatography after successful confirmation by SDS-PAGE, and its effectiveness against V. harveyi was evaluated which showed RPS 75 respectively. Our results suggest that OmpK of V. harveyi could be used as a potential protective candidate for L. vannamei against V. harveyi infection. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810203771 | |
| dc.publisher | KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR | |
| dc.sub | Fisheries | |
| dc.theme | Fisheries | |
| dc.these.type | M.F.Sc | |
| dc.title | EVALUATION OF OUTER MEMBRANE PROTEIN K (OMPK) IN PROTECTING LITOPENAEUS VANNAMEI AGAINST VIBRIO HARVEYI INFECTION | |
| dc.type | Thesis |