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Central Agricultural University, Pasighat

The Central Agricultural University has been established by an act of Parliament, the Central Agricultural University Act, 1992 (No.40 of 1992). The Act came into effect on 26th January, 1993 with the issue of notification by the Department of Agricultural Research and Education (DARE), Govt. of India. The University became functional with the joining of first Vice-Chancellor on 13th September, 1993. The jurisdiction of the University extends to six North-Eastern Hill States viz., Arunachal Pradesh, Manipur, Meghalaya, Mizoram, Sikkim and Tripura, with headquarters at Imphal in Manipur. College of Horticulture & Forestry, a constituent College of the Central Agricultural University, Imphal, Manipur, was established on 7th March 2001 at Pasighat, Arunachal Pradesh on the bank of beautiful river Siang.

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  • ThesisItemOpen Access
    Evaluation of Dolichos Bean [Lablab purpureus (L.) Sweet] Genotypes against Aluminium Toxicity
    (College of Horticulture and Forestry, Central Agricultural University, Pasighat, 2017-07-25) Ansari, Mohd. Talha; Warade, S. D..; Mailappa, A.S.
    The present investigation was carried out in the year 2016-2017 in naturally ventilated polyhouse of Vegetable Experimental Farm, Department of Vegetable Science, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh. The experiment was laid out in Factorial Completely Randomized Design with 2 factors (twenty dolichos bean genotypes with four aluminium (Al) level i.e. 0, 15, 30, 45 ppm) and three replications. Individual main effect and their interaction effects were studied for vine length at weekly interval (2nd -4th), morpho-physiological characters (biomass, root length, root tolerance index, root dry matter, shoot dry matter, leaf dry matter, total dry matter, uptake of P and Al), quality parameters (total protein, proline and total chlorophyll), parameters of soil (pH, EC, available P and extractable Al) at 4th week after sowing whereas yield parameters (pod weight, pod length, pod width, no of seed per pod and yield) at harvesting. In the present study, genotypes of dolichos bean exhibited significant differences for all characters. Aluminium treatment also expressed significant differences for all characters. However, interaction effect was found significant for all studied characters except vine length at all stages. Vine length at weekly interval, biomass, root length, root dry matter, shoot dry matter, leaf dry matter, total dry matter, uptake of phosphorus reduced significantly due to Al treatment except Al uptake. There was significant increase in total protein and proline content of plants at 4th week due to Al treatment, whereas significant reduction in chlorophyll content was observed. In soil parameters, pH and available P decreased with Al treatment, while EC and extractable Al increased significantly with respect to control. All the yield parameters were significantly reduced due to Al treatment. At lower concentration (15 ppm) there was no significant effect on vine length and yield parameters whereas morpho-physiological, soil and quality parameters were significantly affected. At higher concentrations (30 and 45 ppm) all the parameters were significantly influenced. From the present study it can be concluded that G1, G2, G4, G5, G10 and G16 are tolerant based on morpho-physiological characteristic and root tolerance index. Genotypes G1, G2, G4, G11 and G18 were found superior for yield characteristics whereas, G4 and G5 were superior for quality parameters. Overall genotypes G1 (Pusa Sem 3), G2 (VRSEM-207), G4 (VRSEM-601) were found tolerant to aluminium toxicity as well as high yielding.
  • ThesisItemOpen Access
    Effect of bio-fertilizer on growth and yield of cowpea [Vigna unguiculata (L.) Walp] var. Kashi Kanchan under foot hills of Arunachal Pradesh.
    (College of Horticulture and Forestry, Central Agricultural University, Pasighat, 2015-11-17) Nadeem, Mohammad Arshad; Singh, Vikas; Singh, Barun.
    The present investigation was carried out during zaid in 2014 at the Experimental Farm, Department of Vegetable Science, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh. The experiment was laid out in Factorial Randomized Block Design with 12 treatments, three levels of P (0, 20 and 40kg/ha) and four levels of bio-fertilizer (Control, Rhizobium @ 10ml/ kg seed, PSB @ 10ml/ kg seed and Rhizobium + PSB each @ 10ml/ kg seed) with three replications. The uniform doses of N and K @ 20 kg/ha along with 10 t/ha FYM were applied to all the treatments. Main effect of phosphorus, bio-fertilizer and their interaction effects were studied for days to 50% germination, days to 1st flowering, plant height, number of leaf per plant, leaf area index(LAI), stem girth, number of branches per plant, root length, number of nodules per plant, total dry matter production, pod yield, physio-chemical properties of soil (pH, , available N, P, K and organic carbon) before sowing and after harvesting and uptake of N, P, and K by plant. In the main effect of phosphorus, treatment P40 (20 : 40 : 20 kg NPK/ha + 10 t/ha FYM) showed the significantly superior performance with respect to almost all the growth parameters, yield, available soil nutrient and nutrient uptake by plant, except days to 50% germination, days to 1st flowering, plant height (at 15 and 30 DAS), number of leaves (at all the stages) and stem girth (at 30 DAS). Among the bio-fertilizers, the effect of B3 (inoculation of seed with Rhizobium + PSB along with application of 20 kg N, 20 kg K2O and 10 FYM/ha.) was found significantly superior for all the growth parameters, yield, available soil nutrient and nutrient uptake by plant, except days to 50% germination, days to 1st flowering, plant height (at 15 and 30 DAS), number of leaves (at all the stages), stem girth (at 30 DAS) and number of branches per plant (at 45 DAS). In interaction effect, P40B3 (inoculation of seed with Rhizobium + PSB along with application of 20 : 40 : 20 kg NPK/ha + 10 t/ha FYM) proved to be superior for all the growth parameters at later stages (45th and/or 60th DAS) of crop growth, while it was statistically at par with treatment P20B3 (inoculation of seed with Rhizobium + PSB along with application of 20 : 20 : 20 kg NPK/ha + 10 t/ha FYM) in most of the growth parameters. The treatment combination, P40B3 also recorded significantly higher green pod yield (196.4 g/plant, 10.64kg/plot and 120.90 q/ha) including available soil nutrient status viz., pH (6.20), N (370.89 kg/ha), P (38.57 kg/ha), K (168.77 kg/ha) and organic carbon (2.8%) and NPK uptake by plant after harvesting. Hence, the treatment P40B3 (application of 20 : 40 : 20 kg NPK/ha + 10 t/ha FYM along with inoculation of seed with Rhizobium + PSB) was considered the best treatment among the other treatments with respect to growth and yield parameters along with physio-chemical properties of soil and nutrient uptake by plant in cowpea variety Kashi Kanchan.
  • ThesisItemOpen Access
    Studies on Heterosis and Wilt Resistance in Brinjal (Solanum melongena L.).
    (College of Horticulture and Forestry, Central Agricultural University, Pasighat, 2016-09-07) Yadav, Pradeep kumar; Warade, S. D.; Hazarika, B.N.
    The present investigation was conducted during Rabi, 2015-2016 at Vegetable Experimental Farm, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh with 8 selected brinjal parental lines namely Swarna Pratibha, NDB-3, Pant Rituraj, Pusa Purple Long, BR-112, CHFB-6, CHFB-7 and Solanum gilo that were crossed in all possible combinations in diallel technique excluding reciprocals [n(n-1)/2]. The resultant 28 F1 hybrids and 8 parents were studied for thirteen characters viz., plant height, number of branches per plant, days to first flowering, days to first fruit harvest, fruit length, fruit girth, fruit yield per plant, fruit yield tones per hacter, number of seeds per fruit, resistance against wilt, solasodine content, total phenol content and anthocyanin content. Data were analyzed statistically for analysis of variance, combining ability, heterosis and gene action. Analysis of variance revealed significant differences among treatments and hybrids for all the characters. Significant variances were observed for general as well as specific combining ability for all the thirteen traits implies that the both additive and non-additive components of heritable variance are responsible for variation. The ratio of GCA variance (σ2 GCA) to SCA variance (σ2 SCA) was lower than unity for all the traits indicating preponderance of non-additive gene action for all traits. Based on GCA effects parents CHFB-6, NDB-3 and BR-112 for earliness; Swarna Pratibha and CHFB-6 for fruits yield; CHFB-6 for anthocyanin and BR-112 for total phenol & solasodine content and CHFB-6 for earliness and fruit yield, Swarna Pratibha, Solanum gilo and Pant Rituraj for wilt resistance. On the basis of SCA effects, crosses Pant Rituraj x CHFB-6,, Pant Rituraj x CHFB-6, Pant Rituraj x CHFB-7, Swarna Pratibha x NDB-3 and Swarna Pratibha x Pusa Purple Long were the most promising for improvement of most of traits including fruit yield and quality. Heterosis studies indicated that heterosis for yield per plant ranged from -28.46 % to 108.88 % over better parent and from -38.75% to 97.92 % over standard variety, Pant Rituraj. The crosses CHFB-7 x Solanum gilo, Pant Rituraj x CHFB-6, and Swarna Pratibha x CHFB-6 showed significant heterosis over better and standard parent for fruit yield. Higher value of 1 and 2 compared to , significance value of h2, average degree of dominance ( / ) ½ and ratio of KD/KR showed the preponderance of dominance genes in the expression of yield and other traits including quality parameters, suggested that heterosis breeding approaches might be more rewarding than selection to improve yield together with quality traits in brinjal. For wilt resistance on the basis of rating scale, out of thirty six genotypes including parents and hybrids, thirteen genotypes were found to be resistance, fifteen were moderately resistance, seven were moderately susceptible and one was susceptible genotype and no genotypes observed to be highly resistance and highly susceptible to wilt disease. SDS-PAGE analysis showed considerable variation in band number of protein in between the parents and hybrids which is ranged from 8-33. In the crosses where Swarna Pratibha, NDB-3, Pant Rituraj and CHFB-6 is used as female parents, their hybrids were found to be closely related to them as compared to their male parents, whereas when Pusa Purple Long, BR-112 and CHFB-7 were used as female parents, their hybrid were more closely related to their male parents.
  • ThesisItemOpen Access
    Studies on genetic diversity among indigenous landraces of cucumber (Cucumis sativus L.) of North Eastern India through morphological traits and seed protein profiling
    (College of Horticulture and Forestry, Central Agricultural University, Pasighat, 2017-07-25) Chakraborty, Dhiman; Warade, S. D.; Wangchu, L.
    Analysis of variance revealed significant differences among the genotypes for all the characters studied. High PCV and GCV, heritability and genetic gain were observed for average fruit weight, fruit yield per plant, number of fruits per plant, fruit length, number of branches per plant, number of seeds per fruit and 100 seed weight. Correlation studies indicated that fruit yield per plant was positively and significantly correlated with average fruit weight, fruit length, fruit diameter and number of seeds per fruit which indicated the importance of these traits in selection for yield. Path analysis revealed that maximum positive direct effect on fruit yield per plant was imposed by average fruit weight followed by number of fruits per plant, node bearing first pistillate flower, days to first pistillate flower anthesis, 100 seed weight and total soluble solids at genotypic level. This indicated that these are the real independent characters and have maximum contribution towards increase in fruit yield per plant. Divergence study revealed average fruit weight contributed maximum per cent to the diversity followed by 100 seed weight, number of branches per plant, fruit yield per plant, fruit diameter and days to first harvest. Maximum inter cluster distance was observed between cluster IV and VI which indicated that the genotypes within these clusters were highly divergent. SDS-PAGE analysis showed considerable variation in band number of protein which ranged from 12-30. Protein banding profile showed that the genotype CHFC-4 was most distantly related to CHFC-26. Hence, it was recommended that these two genotypes could be utilized for crossing programme to create more genetic diversity. SDS-PAGE marker data provided more sub groupings and revealed higher amount of diversity as compared to morphological data in present study.
  • ThesisItemOpen Access
    Studies on Genetic Diversity and Seed Protein Profiling in Brinjal (Solanum melongena L.).
    (College of Horticulture and Forestry, Central Agricultural University, Pasighat, 2016-08-21) Vanlalnunpuia, Vanlal; Warade, S. D.; Singh, Barun
    Data were analyzed statistically for phenotypic and genotypic variance, coefficient of variation, heritability, genetic advance, genetic gain, correlation coefficient, path coefficient, genetic divergence and seed protein banding pattern. Analysis of variance revealed significant differences among the genotypes for all the characters. High PCV and GCV, heritability and genetic gain were observed for plant height, plant breadth, days to 1st harvesting, number of fruits per plant, fruit weight, solasodine and phenols content. Correlation studies indicated that fruit yield per plant was positively and significantly correlated with fruit weight, fruit length and fruit breadth but negative and significant association was established with 50% germination, days to 1st flowering, days to 50% flowering, days to 1st harvesting and number of fruits per plant. Maximum positive direct effect on fruit yield per plant was imposed by number of primary branches per plant followed by fruit length, days to 1st flowering, phenols content, days to 50% flowering, number of fruits per plant, plant breadth, solasodine content, fruit breadth, reducing sugar and fruit weight. However, high negative direct effect was observed for plant height, days to 1st harvesting and days to 50% germination at genotypic level. Divergence study revealed solasodine content contributed maximum percent to diversity followed by reducing sugar, phenols content, number of fruits per plant and fruits yield per plant. Maximum inter cluster distance was observed between cluster II and V and indicated that the genotypes within these were highly divergent. SDS-PAGE analysis showed considerable variation in band number of protein which ranged from 20-48. Protein banding profile showed that the genotype CHFB-1 (Swarna Pratibha) was most distantly related to CHFB-3 ((A landrace of Balek, Arunachal Pradesh). Hence, it was recommended that these two genotypes (CHFB1 and CHFB-3) could be utilized for crossing programme to create more genetic diversity. SDS-PAGE marker data provided more sub groupings and revealed higher amount of diversity as compared to morphological data in present study.
  • ThesisItemOpen Access
    Response of Capsicum (Capsicum annuum L. var. grossum) To Different levels of spacing and training under polyhous condition.
    (College of Horticulture and Forestry, Central Agricultural University, Pasighat, 2015-06-02) Sangama, S. Salsara; Warade, S. D.; Hazarika, B.N.
    The performance of the two varieties indicated that, capsicum hyb. Mahesh No. 7 responded well to plant height (92.2 cm), number of leaves per plant and fruit quality characteristics and hyb. Shabnam to leaf area index (LAI), number of fruits per plant (9.0), reproductive and yield parameters (0.71 Kg/m2). There was no significant differences among two hybrid varieties for number of branches, fruit set percentage and average weight of fruit. The lowest spacing S3 resulted in maximum plant height (93.2 cm), LAI, earliness in harvesting and total yield ( 0.74 Kg/m2) and highest spacing S1 resulted in maximum number of branches, number of leaf, earliness in fruit flowering and fruit set. There was no positive effect on fruit set %, fruits per plant, average weight of fruit and yield per plant due to spacing levels. Among the training levels, T1 sowed maximum plant height (89.9 cm), minimum days required to first flowering, first fruit set, days to first harvest and size of fruit and T3 resulted in maximum number of branches, number of leaf, LAI, fruits per plant (10.06), yield per plant (0.54 kg) and yield (0.75 Kg/m2). The different levels of spacing and training had no positive effect on shelf life of fruits. However, the fruits still remained marketable upto 8-9 days when stored at room temperature. It is concluded that for higher yield of capsicum under polyhouse conditions, the spacing of 50x40 cm with four shoots training maybe suggested for var. Shabnam in foot hills of Himalayas.
  • ThesisItemOpen Access
    Studies on Nutritive and Anti-nutritive Components of Important Underutilized Perennial Vegetables of Arunachal Pradesh (North East Region of India)
    (College of Horticulture and Forestry, Central Agricultural University, Pasighat, 2017-08-25) Bui, P. Mary; Warade, S. D.; Tayeng, Tissu
    The analysis of nutritional compositions exhibited that the maximum reducing sugar content was found in Houtttuynia cordata (11.15+ variance 0.02%), total carbohydrate content in leaves of moringa oleifera (40.43+0.02 g/100 g), starch content in Sauropus androgynus (1227.96+0.02 mg/100g),Cellulose contentd in Sauropus androgynus (332.05+0.02 mg/100g), crude fibre content in Litsea cubeba (43.41+0.03%), free fatty acid content in the leaf of Moringa oleifera (5.61+0.01 Mg/KOHg). The highest total free amino acid content was found in Passifora edule (30.54+0.02mg/100 g), protein content in Passiflora edule (45.73+0.02 umole/g), methionine content in Moringa oleifera (1.90+0.02 mg/g), crude protein content in Passiflora edule (7.88+0.02%), thiamine content in Polygonum chinense (47.97+0.03 mg/100g), vitamin C content in Passiflora edulis (329.87+0.02 mg/100g) and vitamin E content in Elatostema sessile (15.99+0.01 mg/100g). The highest chlorophyll a content was in the leaf Moring oleifera (0.40+0.02 mg/g), chlorophyll b and total chlorophyll content in Pouzolzia viminea were (0.65+0.02 mg/g) and (0.99+0.01 mg/g) respectively, carotenoids condtent in Passiflora edulis (22.08+0.01mg/100g). The N contentwas found highest in Pssiflora edulis (12.61+0.03 g/kg) whereas, other minerals like P, K and Na was found highest in malebud of Musa arunachalensis (0.76+0.01%), (8.00+0.01 mg/g) and (13.50+0.03 ppm) respectively. Iron content was found highest in Eryngium foetidum(9.2+0.03 mg/100g). The analysis of antinutritive components revealed that the highest cynogen content was found in Bambusa sp. (65.52+0.02 mg/100g) and lowest in Polygonu chinense (5.07+0.01 mg/100g), highest phytic acid content in Sauropus androgynus (735+0.02 mg/100g) and least in Pouzolzia viminea (57.00+0.03mg/100g), highest tannin content in Polygonum chinense (196+0.01 mg/100g) and least in Houttuynia cordata (23.52+0.03mg/100 g). From the above 24 perennial vegetable taken for analysis, leaves of Moringa oleifeara, Sauropus androgynus, Passiflora edulis, Piper pedicellatum, malebud of Musa arunachalensis and polygnum chinense were found to be rich in nutritional components with very low concentration of anti-nutritional components. But slightly higher concentration of anti-nutritional components were found in Moringa oleifera leaves, Sauropus androgynus and Passiflora edulis. The research work performed confirms that the wild edible plants are rich source of nutrient, vitamin and mineral supplements for indigenous tribal population and hence, reduce the vulnerability of local communities to nutrition food scarcity
  • ThesisItemOpen Access
    Screening of cowpea [Vignaunguiculata (L.) Walp] genotypes for aluminium toxicity.
    (College of Horticulture and Forestry, Central Agricultural University, Pasighat, 2014-10-18) Kushwaha, Jitendra Kumar; Warade, S. D.; Pandey, A.K.
    The present investigation was carried out in the year 2013 in the Experimental Farm, Department of Vegetable Science, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh. The experiment was laid out in Factorial Randomized Block Design with 80 treatments (twenty cowpea genotypes with four aluminium level i.e.0, 20, 40, 60 ppm) and three replications. Individual main effect and their interaction effects were studied for plant height at weekly interval, for biomass, number of nodules per plant, root length, shoot length, root dry matter, shoot dry matter, leaf dry matter, total dry matter, uptake of Aluminium and Manganese by plant in root and shoot at 5 week after planting, for number of pods per plant, pod weight, yield at harvesting, total protein content, seed protein profiling and post cropping parameters of soil (pH, available P, extractable Al, extractable Mn). In the present study, genotypes of cowpea exhibited significant differences for all characters. Aluminium treatment also shows significant differences for all character except biomass per plant. However, interaction effect was found significant for all studied character except plant height at all stages and manganese content in soil. Based on mean performance the genotypes G-15, G-13 and G-2 were found to be superior for the most of the plant characteristics suggesting that they can be used for developing aluminium toxicity tolerant genotype. Similarly, G-7, G-4 and G-12 were found to be superior for yield components. So, these genotypes were promising and may be used as parental source in any breeding programme. Among all aluminium treatment 20 ppm was best for plant characteristics whereas pod yield, protein content and soil parameters were best under control (0 ppm). SDS-PAGE data analysis provides information that protein banding pattern could not be affected by aluminium treatment in soil.
  • ThesisItemOpen Access
    Studies on Genetic variability in chilli (Capsicum annum L.) germplasm through morphological and biochemical markers
    (College of Horticulture and Forestry, Central Agricultural University, Pasighat, 2015-06-25) Alice, Athiko Kaiya; Singh, Vikas; Singh, Barun
    Analysis of variance revealed that significant difference among the genotypes for all the characters. High PCV and GCV, heritability and genetic gain were observed for all the characters except days to 50% flowering. Correlation studies indicated that green fruit yield per plant was positively and significantly correlated with weight of green fruit, weight of ripe fruit, weight of dry fruit, fruit length,fruit girth , fruit pedicel length, number of seed per fruit and dry fruit yield per plant but negative and significant association was established with days to 50% flowering and capsaicin content. Maximimum positive direct effect on green fruit yield per plant was imposed by dry fruit yield per plant, weight of green fruit, fruit length and ascorbic acid content. However, high negative direct effect was observed for weight of dry fruit. Divergence study revealed that number of seed per fruit contributed maximum percent to diversity followed by fruit length, fruit pedicel length, capsaicin content, fruit girth and weight of ripe fruit. Maximum inter cluster distance was observed between cluster IV and VI and indicated that the genotypes within these were highly divergent. SDS-PAGSE analysis showed considerable variation in band number of protein which ranged from 8-18. Protein banding profile showed that the genotype CHFC-50 (A landrace of Pasighat, Arunachal Pradesh) was most distantly related to CHFC-52 (A landrace of Maram, Manipur). Hence, it was recommended that these two genotypes (CHFC-50 and CHFC-52) could be utilized for crossing programme to create more genetic diversity. SDS-PAGE marker data provided more sub grouping and revealed higher amount of diversity as compared to morphological data in present study.