Studies on Genetic Diversity and Seed Protein Profiling in Brinjal (Solanum melongena L.).
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Date
2016-08-21
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College of Horticulture and Forestry, Central Agricultural University, Pasighat
Abstract
Data were analyzed statistically for phenotypic and genotypic variance, coefficient of variation, heritability, genetic advance, genetic gain, correlation coefficient, path coefficient, genetic divergence and seed protein banding pattern.
Analysis of variance revealed significant differences among the genotypes for all the characters. High PCV and GCV, heritability and genetic gain were observed for plant height, plant breadth, days to 1st harvesting, number of fruits per plant, fruit weight, solasodine and phenols content.
Correlation studies indicated that fruit yield per plant was positively and significantly correlated with fruit weight, fruit length and fruit breadth but negative and significant association was established with 50% germination, days to 1st flowering, days to 50% flowering, days to 1st harvesting and number of fruits per plant.
Maximum positive direct effect on fruit yield per plant was imposed by number of primary branches per plant followed by fruit length, days to 1st flowering, phenols content, days to 50% flowering, number of fruits per plant, plant breadth, solasodine content, fruit breadth, reducing sugar and fruit weight. However, high negative direct effect was observed for plant height, days to 1st harvesting and days to 50% germination at genotypic level. Divergence study revealed solasodine content contributed maximum percent to diversity followed by reducing sugar, phenols content, number of fruits per plant and fruits yield per plant. Maximum inter cluster distance was observed between cluster II and V and indicated that the genotypes within these were highly divergent.
SDS-PAGE analysis showed considerable variation in band number of protein which ranged from 20-48. Protein banding profile showed that the genotype CHFB-1 (Swarna Pratibha) was most distantly related to CHFB-3 ((A landrace of Balek, Arunachal Pradesh). Hence, it was recommended that these two genotypes (CHFB1 and CHFB-3) could be utilized for crossing programme to create more genetic diversity. SDS-PAGE marker data provided more sub groupings and revealed higher amount of diversity as compared to morphological data in present study.
Description
The present investigation was conducted during February-August 2015 at Vegetable Research Farm, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh in 20 diverse genotypes of brinjal. The experiment was laid out in Randomized Block Design with three replications. Genetic variability was studied for characters viz., plant height (cm), number of primary branches per plant, plant breadth (cm), days to 50% germination, days to 50% flowering, days to 1st harvesting, number of fruits per plant, fruit weight (g), fruit length (cm), fruit breadth (cm), fruit yield per plant (kg), solasodine content (mg/100 g fruit), phenols content (mg/100 g) and reducing sugar (%).
Data were analyzed statistically for phenotypic and genotypic variance, coefficient of variation, heritability, genetic advance, genetic gain, correlation coefficient, path coefficient, genetic divergence and seed protein banding pattern.
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