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Thesis Type: M.V.Sc. ×

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    ThesisItemOpen Access
    EFFECT OF SUPPLEMENTING MANNAN OLIGOSACCHARIDE ON THE PERFORMANCE OF MUZAFFARNAGARI SHEEP
    (SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2021-10) KAUSHLENDRA NARAYAN DWIVEDI; Dr. Debashis Roy
    Department of Animal Nutrition SardarVallabhBhai Patel University of Agriculture and Technology, Meerut Student Name : Kaushlendra Narayan Dwivedi ID No. : 4878 Advisor : Dr. Debashis Roy Degree :MVSc (Animal Nutrition) Thesis Title: “Effect of supplementing mannan oligosaccharide on the performance of Muzaffarnagari sheep” ABSTRACT 18 Muzaffarnagari sheep of similar age group were taken from Livestock farm complex II (LFC II), SVPUAT. The animals were separated randomly into 3 groups. 1st group was treated as control. 2nd and 3rd groups were taken as treatment group. 2nd and 3rd groups were supplemented with mannan oligosaccharide at the rate of 2% and 4% on dry matter basis, respectively along with basal diet. The basal diet comprised of wheat straw, chaffed green berseem fodder, jowar fodder and compounded concentrate mixture at a ratio of 30:15:15:40. Concentrate mixture was prepared by mixing barley grain, wheat grain, oat grain, wheat bran, gram chunni, mustard oil cake and mineral mixture in 20, 20, 10, 10, 10, 28 and 2 parts, respectively.Fortnightly body weight, dry matter intake, weight gain, ADG, FCR and faecal scorewererecorded during the trial period.Blood samples were also collected at 30 days interval i.e. day 0, 30, 60, 90 post supplementation. Haematological parameters were analysed in fresh blood samples. Blood biochemical parameters are estimated in blood plasma. The average BW and metabolic BW of all the fortnights were not significantly different (P>0.05) between groups. The overall gain (kg) and daily weight gain (g/d) was found significantly higher (P<0.01) in both the treatment groups than control. T1 group of animals showed higher BCS than control in fourth, fifth and sixth fortnight. The overall BCS was found higher in T1 group than control. Treatment groups were found to have lower faecal score than control.Digestibility of different nutrients was found similar. Digestible DM, OM, EE, NFE, CF, NDF and ADF intake were found similar in all the three groups of experimental animals. Dry matter intake (%BW) was higher in T1 than control. But total DM intake (kg/d) was found similar. TDN (g/kg W 0.75) intake similarly found similar in all the three groups.Overall Plasma cholesterol concentration was found lower in T2 group than control. But, HDL cholesterol value of T1 and T2 groups were found similar to control throughout the trial period. Plasma TIg concentration was found to increase in T2 group than other. BUN and creatinine concentration of all treatment groups was found similar with control. Liver enzyme activity was also found similar as the activity of ALT, AST and ALKP remained similar throughout the trial period. Thio barbituric acid reactive substance activity of T2 group was found lower than others but plasma FRAP value in present study was found similar in all the experimental groups. In conclusion, Mannan oligosaccharide up to the dose rate of 4% of dry matter improved growth performance, immune parameters, feed intake and feed efficiencyof Muzaffarnagari sheep without adversely affecting blood metabolites and liver function. (Debashis Roy) (Kaushlendra Narayan Dwivedi) Advisor Autho
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    ThesisItemOpen Access
    FORMULATION OF AREA SPECIFIC MINERAL MIXTURE FOR MEERUT DISTRICT AND EFFECT OF ITS SUPPLEMENTATION ON THE PERFORMANCE OF BUFFALO HEIFERS
    (SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2021) VAIBHAV ARYA; Dr. Debashis Roy
    Department of Animal Nutrition Sardar VallabhBhai Patel University of Agriculture and Technology, Meerut Student Name : Vaibhav Arya ID No. : 3139 Advisor : Dr. Debashis Roy Degree : MVSc (Animal Nutrition) Thesis Title: “Formulation of area specific mineral mixture for Meerut district and effect of its supplementation on the performance of buffalo heifers” ABSTRACT Present study was conducted to formulate the area specific mineral mixture (ASMM) for Meerut district and its subsequent supplementation to buffalo heifers to observe the effect on performance and blood biochemical parameters. Total 140 feed samples, 120 fodder samples, 105 water samples, 58 milk samples, 51 blood samples and 29 soil samples were collected randomly from all the 12 blocks of Meerut districts. After processing, the samples were analysed for different macro and micro elements by Inductively Coupled Plasma (ICP) instrument (instrument name MY2015CQ05 of Agilent Technologies). Among the feeds, wheat bran was found deficient in copper by 15%. Both copper and zinc content in Mustard oil cake was found marginal. Copper was deficient in bajra fodder, berseem fodder and jowar fodder by 7.5%, 30% and 29.3%, respectively. Sugercane top, bajra fodder, berseem fodder and jowar fodder were found deficient in zinc by 7.3%, 14.67%, 5% and 2%, respectively. Copper and zinc were also found deficient in blood sample by 15.38% and 1.25%, respectively whereas animal milk samples were deficient in copper by 66.67%. Thus, an ASMM was formulated which was fortified with extra copper and zinc over and above BIS type I specification. 18 Murrah buffalo heifers were randomly divided into three groups (T1, T2 and T3) of six animals each on the basis of body weight. Control or T1 groups of animals were fed with basal diet composed of wheat straw, chaffed green berseem fodder and compounded concentrate mixture at a ratio of 35:40:25 with no mineral mixture supplementation. T2 group of animals were supplemented with ASMM whereas T3 group of animals were supplemented with BIS type I mineral mixture along with basal diet. Growth performance, haematological and blood biochemical parameters were analysed. Fortnightly bodyweight gain and average daily gain were found significantly higher (P<0.05) in ASMM and BIS type I mineral mixture supplemented animals than control. Body condition score was found significantly higher in ASMM supplemented animals than control. Digestibility and digestible nutrient intake of crude protein, ether extract, nitrogen free extract, neutral detergent fibre and acid detergent fibre were also not affected by supplementing mineral mixture. Average haemoglobin, PCV, RBC, Lymphocyte and monocyte (%) value increased in mineral supplemented animals than control. TLC and neutrophil (%) reduced in mineral supplemented animals than control. Plasma glucose, total protein, albumin and total immunoglobulin values were found to be more in mineral supplemented animals. Antioxidant assay revealed that super oxidase dismutase, catalase and total antioxidant activities were higher in mineral supplemented animals than control. Thio-barbituric acid reactive substance (TBARS) activity was decreased in mineral supplemented animals than control. In conclusion, ASMM prepared for Meerut district by fortifying copper and zinc improved growth performance, haematological status and antioxidant activities of Murrah buffalo heifers without adversely affecting blood metabolites and liver function. (Debashis Roy) (Vaibhav Arya) Advisor Author
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    ThesisItemOpen Access
    ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF CAPRINE FAECAL ORIGIN LACTOBACILLI AS POTENTIAL PROBIOTIC FOR IMPROVING NUTRIENT UTILIZATION, HEALTH AND PRODUCTIVITY IN GOATS
    (SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2021-09) PRASHANT PANDEY; Dr. Ajit Kumar
    Name :Prashant Pandey I.d. No. : 3093 Advisor : Dr. Ajit Kumar Degree : M.V.Sc (Animal Nutrition) Department :Animal Nutrition Thesis Title: “Isolation, Identification and Characterization of Caprine Faecal Origin Lactobacilli as Potential Probiotic for Improving Nutrient Utilization, Health and Productivity in Goats” ABSTRACT The health of goat farming is closely related to how the animals are raised and the gut microbiota plays a key role in the fulfilment of their productive potential. The objective of the present study was to isolate and characterize a lactobacilli strain of caprine origin from the goat faeces and assess its efficacy in-vitro as potential probiotics for goat nutrition. A total of forty two fresh faecal samples from different healthy goats were aseptically collected. The enrichment and culture of the samples was done on selective culture medium of de Man Rogosa and Sharpe broth and agar (MRS). Then two selected bacterial isolates were purified and conventionally characterized based on the morphological and biochemical analysis such as gram staining, catalase test, motility test, endospore and carbohydrate fermentation tests. All the isolates were either rod or cocci shaped, gram positive, catalase negative, non-motile and non-spore formers. On the sugar fermentation test the isolates showed positive against cellobiose, galactose, lactose, maltose, mannose and sucrose while all isolates were negative for mannitol sugar. On the basis of phenotypic and sugar utilization tests two isolates were shortlisted further by in-vitro testing of aggregation time, cell surface hydrophobicity, acid tolerance etc. to select the high viability strain after encountering likely adverse condition in gastrointestinal tract for use a potential probiotic for goat. The molecular characterization was done on the basis of 16S rDNAand sequencing was done. The two lactobacilli spp. were identified as WeissellaconfusaSVPUAT-1 & SVPUAT-2. The in vivo trail was conducted on goats for evaluation of isolated caprine faecal origin Lactobacilli as probiotics for enhanced nutrient utilization and productivity. Eighteen adult goats were divided into three equal groups and fed on a basal diet supplemented with either no probiotics (CON), or with a probiotic of caprine-origin (Weissellaconfusa; tPRO) or dairy-origin (Lactobacillus acidophilus NCDC15; cPRO). The feed consumption, nutrient intake and FCR remained un-influenced by probiotic treatments. The digestibility of CF, CP and EE in tPRO and cPRO was higher (P<0.05) than CON however digestibility DM, OM and NFE remain same in all groups. Body weight get increased during the trial period but difference was not significant among the groups. Hb and PCV were similar among the groups. The common biochemical parameters of glucose, cholesterol, triglycerides, albumin, globulin, TP, ALT and AST were also found similar (P>0.05) among the groups. Based on all finding, it was concluded that isolated caprine faecal origin lactobacilli bacteriaWeissellaconfusa (Gene Bank Accession No. MZ314055.1) have majority of probiotics characteristics and can be used as probioticsin the diet of goats for enhanced nutrient utilization, health and productivity. (Dr Ajit Kumar) (Prashant Pandey) Advisor Author