ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF CAPRINE FAECAL ORIGIN LACTOBACILLI AS POTENTIAL PROBIOTIC FOR IMPROVING NUTRIENT UTILIZATION, HEALTH AND PRODUCTIVITY IN GOATS
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Date
2021-09
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SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),
Abstract
Name :Prashant Pandey I.d. No. : 3093
Advisor : Dr. Ajit Kumar Degree : M.V.Sc (Animal Nutrition)
Department :Animal Nutrition
Thesis Title: “Isolation, Identification and Characterization of Caprine Faecal Origin Lactobacilli as
Potential Probiotic for Improving Nutrient Utilization, Health and Productivity in Goats”
ABSTRACT
The health of goat farming is closely related to how the animals are raised and the gut microbiota plays a
key role in the fulfilment of their productive potential. The objective of the present study was to isolate
and characterize a lactobacilli strain of caprine origin from the goat faeces and assess its efficacy in-vitro
as potential probiotics for goat nutrition. A total of forty two fresh faecal samples from different healthy
goats were aseptically collected. The enrichment and culture of the samples was done on selective culture
medium of de Man Rogosa and Sharpe broth and agar (MRS). Then two selected bacterial isolates were
purified and conventionally characterized based on the morphological and biochemical analysis such as
gram staining, catalase test, motility test, endospore and carbohydrate fermentation tests. All the isolates
were either rod or cocci shaped, gram positive, catalase negative, non-motile and non-spore formers. On
the sugar fermentation test the isolates showed positive against cellobiose, galactose, lactose, maltose,
mannose and sucrose while all isolates were negative for mannitol sugar. On the basis of phenotypic and
sugar utilization tests two isolates were shortlisted further by in-vitro testing of aggregation time, cell
surface hydrophobicity, acid tolerance etc. to select the high viability strain after encountering likely
adverse condition in gastrointestinal tract for use a potential probiotic for goat. The molecular
characterization was done on the basis of 16S rDNAand sequencing was done. The two lactobacilli spp.
were identified as WeissellaconfusaSVPUAT-1 & SVPUAT-2. The in vivo trail was conducted on goats
for evaluation of isolated caprine faecal origin Lactobacilli as probiotics for enhanced nutrient utilization
and productivity. Eighteen adult goats were divided into three equal groups and fed on a basal diet
supplemented with either no probiotics (CON), or with a probiotic of caprine-origin (Weissellaconfusa;
tPRO) or dairy-origin (Lactobacillus acidophilus NCDC15; cPRO). The feed consumption, nutrient intake
and FCR remained un-influenced by probiotic treatments. The digestibility of CF, CP and EE in tPRO and
cPRO was higher (P<0.05) than CON however digestibility DM, OM and NFE remain same in all groups.
Body weight get increased during the trial period but difference was not significant among the groups. Hb
and PCV were similar among the groups. The common biochemical parameters of glucose, cholesterol,
triglycerides, albumin, globulin, TP, ALT and AST were also found similar (P>0.05) among the groups.
Based on all finding, it was concluded that isolated caprine faecal origin lactobacilli
bacteriaWeissellaconfusa (Gene Bank Accession No. MZ314055.1) have majority of probiotics
characteristics and can be used as probioticsin the diet of goats for enhanced nutrient utilization, health
and productivity.
(Dr Ajit Kumar) (Prashant Pandey)
Advisor Author