Thumbnail Image

Theses

Browse

 Reset filters

Settings

Subject: Agricultural Microbiology ×

Search Results

Now showing 1 - 9 of 40
  • Thumbnail Image
    ThesisItemOpen Access
    FUNGAL BIOFERTILIZERS FOR PREPARATION OF P AND K RICH ORGANIC MINERAL MANURES AND THEIR EFFICACY IN MAIZE
    (DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2021) Solanki Jayvirsinh Pratapsinh; Dr. R. V. Vyas
    The nutrient pool and soil organisms are highly affected due to declining soil health. Reduction of fertilizer use efficiency is the reflection of injudicious use of agrochemicals for crop nutrition and protection, such activity lead to lower organic carbon content in soil. Restoration of soil health, lowering down chemical fertilizer requirement and enhancement of microbial activity was thought and attempted in present study using native plant growth promoting fungal isolates (PGPF). Fungal cultures were isolated from different AAU farms based on their abilities to solubilize P and K mineral forms. They were further screened for pathogenicity symptoms on seed germination and seedling growth parameters. Two non-pathogenic fungi were identified as isolate T-3, Aspergillus flavipes (NCBI Access No. MW88450) and T-17, Trichoderma aggressivum (NCBI Access No. MW88453).
  • Thumbnail Image
    ThesisItemOpen Access
    EFFICACY OF PESTICIDE DEGRADING NATIVE BACTERIA AS PGPR IN MAIZE (Zea mays L.)
    (DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2021) Patel Tasvirkumar Manubhai; Dr. H. K. Patel
    The study was conducted with the aim to isolate and characterize the plant growth promotion activity of chlorpyrifos, profenofos and atrazine degrading native bacteria. Soil samples were collected from different research farms of Anand Agricultural University, Anand having history of pesticide application. Total 21 bacterial isolates were obtained following enrichment isolation technique from soil samples and screened for their pesticide degradation efficiency. Total 6 isolates were found effective for chlorpyrifos degradation, 6 for profenofos and 9 were found effective for the degradation of atrazine. Among these isolates, 2 best isolates were selected for each pesticide on the basis of their ability to utilize respective pesticide at higher concentration as sole source of carbon and energy. Based on results of the polyphasic characterization isolate CN1 was identified as Pseudomonas nitroreducens AAUCP 1 and CN 3 as Pseudomonas alcaligenes AAU CP 3 fond to degrade chlorpyrifos effectively; profenofos degrader isolate PN3 was identified as Pseudomonas aeruginosa AAUPF 3 and P3 as Pseudomonas stutzeri AAUPF 2; atrazine degrading isolates A2 identified and designated as Paenarthrobacter ureafaciens AAUATR 2 and isolate AN4 as Pseudomonas taiwanensis AAU ATR 4.
  • Thumbnail Image
    ThesisItemOpen Access
    PHYCOREMEDIATION OF HEAVY METALS BY BIOFUEL PRODUCING ALGAE
    (DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2021) Kamaliya Sagar Nanjibhai; Dr. Y. K. Jhala
    Algae are having enormous metabolic potential and diversity, which makes them organisms of interest for exploring as bioremediation of environmental pollutants as well as feedstock for biofuel production. Present investigation was undertaken with the aim to isolate potential strains of microalgae having capacity to remove heavy metals viz. Cadmium (Cd), Cobalt (Co), Chromium (Cr), Nickel (Ni) and Lead (Pb) from water and additional characteristic of high lipid production efficiency.
  • Thumbnail Image
    ThesisItemOpen Access
    ISOLATION AND EFFICIENCY OF NATIVE BACTERIAL AND FUNGAL BIODEGRADERS FOR DECOMPOSITION OF COTTON (Bt) WASTE
    (DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2019) Patel Harshkumar; Dr. R.V. Vyas
    The present investigation was aimed to isolate and identify the native lignocellulose biodegrader microbes for biological degradation of cotton (Bt) waste and convert it into nutrient rich compost. Total 120 bacterial and 30 fungal cultures were isolated from cotton field soil by serial dilution technique and one culture was recovered from termite gut and screened for cellulose degradation ability on carboxy methyl cellulose (CMC), three bacterial and three fungal isolates were selected for further studies based on their highest activity on CMC. The solubilisation index (SI) by bacterial cultures was ranged from 2.74 to 3.49, similarly SI by fungal cultures was between 2.47 to 2.58. Among selected efficient bacterial isolates BDCT-1, 2 and 3 identified by cultural, morphological and molecular characterization (16S rDNA) and found as Pseudomonas stetzuri AAU BDCT 1, Bacillus velezensis AAU BDCT 2 and Bacillus sp. Similarly, among selected efficient fungal isolates FCT-2 and 3 identified as Aspergillus terreus and A. flavus respectively.
  • Thumbnail Image
    ThesisItemOpen Access
    ISOLATION, CHARACTERIZATION AND CO-INOCULATION EFFECT OF ROOT NODULE NON RHIZOBIAL ENDOPHYTES AND Rhizobium IN GREEN GRAM (Vigna radiata L.)
    (DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2019) Archana Maruti Dhole; Mrs. H. N. Shelat
    Present research was carried out to isolate, characterize and investigate Plant Growth Promoting (PGP) traits of Non Rhizobial Endophytes (NRE) and Rhizobium from root nodules of green gram as well as well as to ascertain their co-inoculation effect in pot and field conditions. Totally, 53 bacteria were isolated from root nodule of green gram collected from five different farms of Anand Agricultural University. Based on screening for antifungal activity, phosphate solubilisation, IAA production and ARDRA profile, two NRE and one rhizobial type isolates were selected and identified through partial 16S rRNA gene sequence analysis as Bacillus cereus AAU B6 (MH701892), Bacillus tequilensis AAU B12 (MH701893) and Rhizobium phaseoli AAU B3 (MH701891)
  • Thumbnail Image
    ThesisItemOpen Access
    ISOLATION AND CHARACTERIZATION OF LIGNOCELLULOSE BIODEGRADING BACTERIA FROM BEEJAMRUT AND JEEVAMRUT AND THEIR EFFICACY ON RICE AGRO-WASTE
    (DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2018) Nisarta Roshankumar Sudhakarbhai; Mrs. H. N. Shelat
    Lignocellulose biodegrading bacteria were isolated from the Beejamrut and Jeevamrut on Nutrient agar, L-MSM agar and CMC agar medium by serial dilution technique wherein, the highest microbial population was found to be at first day of preparation from Beejamrut whereas in Jeevamrut it was maximum at 15th day of preparation. Total 48 morphologically distinguished isolated colonies were selected, out of which six isolates, J1, J4, J6, B1, B3 and B5 shown promising zone on CMC agar and five isolates, J2, J3, J5, B2 and B4 growth on L- MSM Lignin agar. Further they were screened for the production of laccase and lignin Peroxidase and found positive. Cultural, morphological, biochemical and molecular characterization through 16S rRNA sequencing was carried out for identification of selected isolates and found belonging to genus Rhodococcus viz. R. equi AAU J1 Accn. No. MH591220, R. pyridinivorans AAU J3 Accn. No. MH591222; genus Psuedomonas viz. P. aeruginosa AAU J2 Accn. No. MH591221, P. punonensis AAU B4 Accn. No. MH591229 and genus Bacillus viz. B. cereus AAU J4 Accn. No. MH591223, B. safensis AAU J6 Accn. No. MH591225, B. safensis AAU J5 Accn. No. MH591224, B. tequilensis AAU B2 Accn. No. MH591227, B. subtilis AAU B3 Accn. No. MH591228, B. zhangzhouensis AAU B5 Accn. No. MH591230 and B. austalimaris AAU B1 Accn. No. MH591226.
  • Thumbnail Image
    ThesisItemOpen Access
    INFLUENCE OF INSECTICIDES, FUNGICIDES AND HERBICIDES ON BIO-NPK BACTERIAL CONSORTIUM IN VITRO AND ON COTTON IN POTS
    (DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2018) Manva Fizanulmustufa Sabirhusen; Dr. R. V. Vyas
    Influence of insecticides, fungicides and herbicides on Bio NPK consortium in vitro and on cotton in pots was studied. In vitro influence of pesticides on Bio NPK consortium and its individual member was tested by liquid and plate bio assay with the three doses of pesticides i.e. field recommended dose (X), half the field recommended dose (½X) and double the field recommended dose (2X). Seven pesticides, five fungicides and three herbicides were taken for laboratory study and found that insecticides viz. Spinosad 45% SC, Fipronil 5% SC, Imidacloprid 30.5% EC and Dichlorvos 76% EC were found compatible at all the three doses in plate assay. While, Profenofos 50% EC, Chlorpyrifos 20% EC, Copper oxychloride 50% W/W, Ridomil 68% WP, Pyraclotrobin 20% WG, Pendimethalin 38.7% EC, Quizalofop 5% EC and Finoxaprop 9% EC, found compatible at regular dose of pesticide.
  • Thumbnail Image
    ThesisItemOpen Access
    PLANT GROWTH PROMOTING RHIZOSPHERIC BACTERIAL ISOLATION, CHARACTERIZATION AND CONSORTIUM DEVELOPMENT FOR POTASH MOBILIZATION AND ITS EFFICACY IN MAIZE (ZEA MAYS L.)
    (DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY, ANAND, 2018) Patel Ankitkumar Subhashbhai; Mrs. H. N. Shelat
    Series of experiments were carried out to explore plant growth promoting abilities of native rhizospheric potash mobilizing bacterial (KMB) isolates. Total 116 bacteria were isolated, wherein 42 isolates showed prominent efficiency of K mobilization, from which, 17 prominent cultures were selected on the basis of the highest zone of solubilization on Aleksandrov agar supplemented with mica. Isolate M-2 gave the highest potash solubilizing index 8.0 and K release 21.41 μg/ml at 4 days after inoculation in Aleksandrov broth. All the 17 KMB isolates exhibited organic acid and capsular polysaccharides production out of which 14 isolates were positive for acidic exo-polysaccharides.
  • Thumbnail Image
    ThesisItemOpen Access
    ISOLATION OF PINK PIGMENTED FACULTATIVE METHYLOTROPHS (PPFMs) FROM THE PHYLLOSPHERE OF SOLANACEOUS CROPS AND ITS EFFICACY ON TOMATO, BRINJAL AND CHILLI
    (B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY, 2018) Solanki Jayvirsinh Pratapsinh; Dr. R. V. Vyas
    Pink Pigmented Facultative Methylotrophs (PPFMs) were isolated from the phyllosphere of solanaceous crops from Anand on NMS medium supplemented with methanol (1%) by leaf imprinting technique. The isolates were checked for survival on methanol 1 to 5%, 22 isolates were survived to 5% methanol and less, on the basis of enzymatic activity of isolates for methane degradation (Soluble methane monooxygenase and methanol dehydrogenase) five isolates were selected. Morphological, Biochemical and Molecular characterization through 16S rRNA sequencing was carried out for identification of selected isolates and all found to belonging genus Methylobacterium viz. Methylobacterium populi AAU PPFM C-7 (MH578621), Methylobacterium radiotolerans AAU PPFM C-17 (MH578622), M. populi AAU PPFM C-19 (MH578623), M. populi AAU PPFM T-2 (MH578624) and M. radiotolerans B-2 (MH586819).