ISOLATION OF PINK PIGMENTED FACULTATIVE METHYLOTROPHS (PPFMs) FROM THE PHYLLOSPHERE OF SOLANACEOUS CROPS AND ITS EFFICACY ON TOMATO, BRINJAL AND CHILLI
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Date
2018
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B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY
Abstract
Pink Pigmented Facultative Methylotrophs (PPFMs) were isolated from the phyllosphere of solanaceous crops from Anand on NMS medium supplemented with methanol (1%) by leaf imprinting technique. The isolates were checked for survival on methanol 1 to 5%, 22 isolates were survived to 5% methanol and less, on the basis of enzymatic activity of isolates for methane degradation (Soluble methane monooxygenase and methanol dehydrogenase) five isolates were selected. Morphological, Biochemical and Molecular characterization through 16S rRNA sequencing was carried out for identification of selected isolates and all found to belonging genus Methylobacterium viz. Methylobacterium populi AAU PPFM C-7 (MH578621), Methylobacterium radiotolerans AAU PPFM C-17 (MH578622), M. populi AAU PPFM C-19 (MH578623), M. populi AAU PPFM T-2 (MH578624) and M. radiotolerans B-2 (MH586819).
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