ISOLATION AND CHARACTERIZATION OF LIGNOCELLULOSE BIODEGRADING BACTERIA FROM BEEJAMRUT AND JEEVAMRUT AND THEIR EFFICACY ON RICE AGRO-WASTE
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Date
2018
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DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND
Abstract
Lignocellulose biodegrading bacteria were isolated from the Beejamrut and Jeevamrut on Nutrient agar, L-MSM agar and CMC agar medium by serial dilution technique wherein, the highest microbial population was found to be at first day of preparation from Beejamrut whereas in Jeevamrut it was maximum at 15th day of preparation. Total 48 morphologically distinguished isolated colonies were selected, out of which six isolates, J1, J4, J6, B1, B3 and B5 shown promising zone on CMC agar and five isolates, J2, J3, J5, B2 and B4 growth on L- MSM Lignin agar. Further they were screened for the production of laccase and lignin Peroxidase and found positive. Cultural, morphological, biochemical and molecular characterization through 16S rRNA sequencing was carried out for identification of selected isolates and found belonging to genus Rhodococcus viz. R. equi AAU J1 Accn. No. MH591220, R. pyridinivorans AAU J3 Accn. No. MH591222; genus Psuedomonas viz. P. aeruginosa AAU J2 Accn. No. MH591221, P. punonensis AAU B4 Accn. No. MH591229 and genus Bacillus viz. B. cereus AAU J4 Accn. No. MH591223, B. safensis AAU J6 Accn. No. MH591225, B. safensis AAU J5 Accn. No. MH591224, B. tequilensis AAU B2 Accn. No. MH591227, B. subtilis AAU B3 Accn. No. MH591228, B. zhangzhouensis AAU B5 Accn. No. MH591230 and B. austalimaris AAU B1 Accn. No. MH591226.
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