ISOLATION AND EFFICIENCY OF NATIVE BACTERIAL AND FUNGAL BIODEGRADERS FOR DECOMPOSITION OF COTTON (Bt) WASTE
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Date
2019
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DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND
Abstract
The present investigation was aimed to isolate and identify the native lignocellulose
biodegrader microbes for biological degradation of cotton (Bt) waste and convert it into
nutrient rich compost. Total 120 bacterial and 30 fungal cultures were isolated from cotton
field soil by serial dilution technique and one culture was recovered from termite gut and
screened for cellulose degradation ability on carboxy methyl cellulose (CMC), three bacterial
and three fungal isolates were selected for further studies based on their highest activity on
CMC. The solubilisation index (SI) by bacterial cultures was ranged from 2.74 to 3.49,
similarly SI by fungal cultures was between 2.47 to 2.58. Among selected efficient bacterial
isolates BDCT-1, 2 and 3 identified by cultural, morphological and molecular
characterization (16S rDNA) and found as Pseudomonas stetzuri AAU BDCT 1, Bacillus
velezensis AAU BDCT 2 and Bacillus sp. Similarly, among selected efficient fungal isolates
FCT-2 and 3 identified as Aspergillus terreus and A. flavus respectively.