ISOLATION, CHARACTERIZATION AND CO-INOCULATION EFFECT OF ROOT NODULE NON RHIZOBIAL ENDOPHYTES AND Rhizobium IN GREEN GRAM (Vigna radiata L.)
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Date
2019
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DEPARTMENT OF AGRICULTURAL MICROBIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND
Abstract
Present research was carried out to isolate, characterize and investigate Plant Growth Promoting (PGP) traits of Non Rhizobial Endophytes (NRE) and Rhizobium from root nodules of green gram as well as well as to ascertain their co-inoculation effect in pot and field conditions. Totally, 53 bacteria were isolated from root nodule of green gram collected from five different farms of Anand Agricultural University. Based on screening for antifungal activity, phosphate solubilisation, IAA production and ARDRA profile, two NRE and one rhizobial type isolates were selected and identified through partial 16S rRNA gene sequence analysis as Bacillus cereus AAU B6 (MH701892), Bacillus tequilensis AAU B12 (MH701893) and Rhizobium phaseoli AAU B3 (MH701891)