Browsing by Author "Satish, A.C."
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ArticleItem Open Access Bunostomum trigonocephalum infection in sheep - Pathological lesions and molecular characterization(2017) Satish, A.C.; Nagarajan, K.; Balachandran, C.; Soundararajan, C.; Sridhar, R.; TANUVASA total of 103 intestines of sheep from slaughter house at Perambur, Chennai, Tamil Nadu were examined for the pathological lesions due to parasitic infections. Hook worm infection due to Bunostomum trigonocephalum could be identified in three animals. Grossly, infected intestine showed multiple petechiae in the ileal mucosa which were visible from serosal surface. Adult parasites were seen attached to the mucosal surface around which petechiae and oedema were noticed. Histopathologically, the haematoxylin and eosin (H&E) stained sections of jejunum revealed desquamation. congestion and haemorrhage with severe infiltration of eosinophils, plasma cells and lymphocytes. For molecular identification of Bunostamum species, polymerase chain reaction (PCR) was carried out targeting ITS2 region. DNA isolated from both the faecal eggs and adult worms showed specific 171 bp sized amplicon.ThesisItem Open Access GASTROINTESTINAL PATHOLOGY OF SMALL RUMINANTS WITH SPECIAL REFERENCE TO PARASITIC DISEASES(TANUVAS, Chennai, 2017) Satish, A.C.; Balachandran, C.; TANUVAS; Balachandran, C.; Soundararajan, C.A total of 344 faecal samples and 145 blood samples were collected from live sheep and goats at large animal clinics (Med), Department of Clinics, Madras Veterinary College (MVC), slaughter houses, private sheep and goat farms of Tiruvallur and Kanchipuram. A total of 125 tissue samples were collected from slaughter house at Chennai, Tiruvallur and Kaneliipuram districts. PCR also performed to know the species of the G1 parasites.ArticleItem Open Access Gross, Histopathology and Molecular diagnosis of oesophagostomosis in Sheep(2018) Satish, A.C.; Nagarajan, K.; Balachandran, C.; Soundararajan, C.; Legadevi, R.; TANUVASA total of 120 tissue samples (Rumen, reticulum, omasum, abomasum, small intestine and large intestine) were collected from slaughter houses in Chennai, Kanchipuram and Tiruvallur districts and five samples from necropsy room at Department of Veterinary Pathology, Madras Veterinary College, Chennai, Tamil Nadu. Overall prevalence of GI parasites was 52.00%. Among the positive samples, single infection was found to be more (53.84%) than themixed infection (46.15%). Out of which, oesophagostomosis was 17.00% and found as single infection. In oesophagostomosis, significant gross lesions observed in the small and large intestine were multiple small to large, round to irregular, hard, fibrotic, raised nodules. The wall of the large intestine was greatly thickened and oedematous and the nodules were seen projecting into the lumen and mucosal surface was intact. On histopathological examination, ileum of sheep showed parasitic nodules with central baso-eosinophilic necrotic area, larval stages in the necrotic area, surrounded by layers of inflammatory cells and finally encapsulated in a thick fibrous connective tissue capsule. Immunohistochemistry (IHC) with Vimentin showed intense cytoplasmic positive signals DAB Brown. PCR was carried out targeting ITS2 regions of the O. columbianum and O. venulosum. DNA isolated from both the faecal eggs and adult worms showed good amplification.ArticleItem Open Access Prevalence of Gastrointestinal Parasites in Small Ruminants in and around Chennai, Tamil Nadu(TANUVAS, 2018-02) Satish, A.C.; Nagarajan, K.; Balachandran, C.; Soundararajan, C.; Arunaman, C.S.; Thangapandian, M.; Sridhar, R.; TANUVASOut of 344 faecal samples collected from sheep (145) and goats (199), 83.43% were found to show gastrointestinal (GI) parasites in and around Chennai, Tamil Nadu. The prevalence of gastrointestinal parasites was almost similar in sheep (83.45%) and goats (83.41%). Mixed infection (58.9%) was more than the single infection (41.1%). Among the single infection, oocyst of Eimeria species (71.18%) was found to be more common followed by Strongyloides spp (13.56%), Strongyle (12.71%), Trichuris spp (1.69%) and amphistomes (0.84%).