Gross, Histopathology and Molecular diagnosis of oesophagostomosis in Sheep
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Date
2018
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Abstract
A total of 120 tissue samples (Rumen, reticulum,
omasum, abomasum, small intestine and large intestine)
were collected from slaughter houses in Chennai,
Kanchipuram and Tiruvallur districts and five samples
from necropsy room at Department of Veterinary Pathology,
Madras Veterinary College, Chennai, Tamil Nadu.
Overall prevalence of GI parasites was 52.00%. Among the
positive samples, single infection was found to be more
(53.84%) than themixed infection (46.15%). Out of which,
oesophagostomosis was 17.00% and found as single
infection. In oesophagostomosis, significant gross lesions
observed in the small and large intestine were multiple
small to large, round to irregular, hard, fibrotic, raised
nodules. The wall of the large intestine was greatly thickened
and oedematous and the nodules were seen projecting
into the lumen and mucosal surface was intact. On
histopathological examination, ileum of sheep showed
parasitic nodules with central baso-eosinophilic necrotic
area, larval stages in the necrotic area, surrounded by layers
of inflammatory cells and finally encapsulated in a thick
fibrous connective tissue capsule. Immunohistochemistry
(IHC) with Vimentin showed intense cytoplasmic positive
signals DAB Brown. PCR was carried out targeting ITS2
regions of the O. columbianum and O. venulosum. DNA
isolated from both the faecal eggs and adult worms showed
good amplification.
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Keywords
Veterinary Science, Veterinary Parasitology