Bunostomum trigonocephalum infection in sheep - Pathological lesions and molecular characterization
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Date
2017
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Abstract
A total of 103 intestines of sheep from slaughter house at Perambur, Chennai, Tamil
Nadu were examined for the pathological lesions due to parasitic infections. Hook worm
infection due to Bunostomum trigonocephalum could be identified in three animals.
Grossly, infected intestine showed multiple petechiae in the ileal mucosa which were
visible from serosal surface. Adult parasites were seen attached to the mucosal surface
around which petechiae and oedema were noticed. Histopathologically, the haematoxylin
and eosin (H&E) stained sections of jejunum revealed desquamation. congestion and
haemorrhage with severe infiltration of eosinophils, plasma cells and lymphocytes. For
molecular identification of Bunostamum species, polymerase chain reaction (PCR) was
carried out targeting ITS2 region. DNA isolated from both the faecal eggs and adult
worms showed specific 171 bp sized amplicon.
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Keywords
Veterinary Science, Veterinary Parasitology