Browsing by Author "Saravanabava, K."
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ArticleItem Open Access Ampli cation of Toll Protein Gene of Penaeus Vannamei, a Commercially Important Penaeid Shrimp(Indian Vet. J., 2013-09) Mahalakshmi, G.; Uma, A.; Rebecca, G.; Saravanabava, K.; TANUVASToll proteins are known to play a key role in innate immune responses in crustaceans like shrimp. The objective of this work was to study the gene of Toll proteins in a commercially important shrimp, Penaeus vannamei (white shrimp) during experimental induction with Vibrio harveyi, a gram-negative bacterial pathogen infecting shrimp.ArticleItem Open Access Antibiotic Resistant Vibrio Harveyi Isolated From Swollen Hindgut Syndrome (Shg) Affected Penaeus Monodon Post Larvae From Commercial Shrimp Hatcheries(TANUVAS, Chennai, 2008-01) Uma, A.; Saravanabava, K.; Singaravel, R.; Koteeswaran, A.; TANUVASThesisItem Open Access Assessment of Haemagglutination Inhibition Assay as a True Measure of Neutralization Titre in Newcastle Disease Virus(TANUVAS, Chennai, 2004) Saravanan, S.; TANUVAS; Saravanabava, K.; Kalaimathi, R.; Dinakaran, A. ManicavasakaThe present study was aimed to assess the influence of three lentogenic Newcastle disease virus (NDV) strains viz B1, LaSota and ‘F’ in haemagglutination inhibition (HI) test. One hundred number day-old Babcock male chicks were divided into four groups of 25 chicks each for B1, LaSota and ‘F’ strains and unvaccinated control. Before vaccination, the maternal antibody titre was assessed. After the maternal antibody titre reached low level, the groups of chicks were vaccinated with respective NDV vaccine strains. The serum samples were collected on 14 and 21 days after homologous booster vaccination and the post vaccinal immune response was assessed by HI test and virus neutralization test (VNT). The HI test was carried out in a manner that each serum sample was titrated against all the three sources of haemagglutinins viz B1, LaSota and ‘F’. Similarly, the VNT was also carried out for pooled serum samples of respective vaccine strains, using the above three antigens. In this study, the maternal antibody mean log2 titre was 5.5 on day one and declined at the rate of one log2 in four days. A protective HI and virus neutralization (VN) titres were observed in all the vaccinated groups and both HI and VN titres were maximum at 21 days post vaccination. There was no significant difference in HI titres using the homologous and heterologous antigens except in the serum samples of the LaSota vaccinated group, a significantly higher titres were observed, when LaSota strain was used as an antigen in HI test. Similarly, no significant difference was observed in VN titres using homologous and heterologous antigens. But, in the LaSota vaccinated group, significantly higher VN titres were observed, while using LaSota as an antigen in VNT. It is concluded that the use of homologous virus in the HI assay after vaccination with LaSota strain lead to an overestimation of protective serum antibody titres. Hence, NDV strains B1 or ‘F’ can be preferred as an antigen in HI assay to measure the serum antibody titre.ArticleItem Open Access Basal Expression of Toll-Like Receptor 18 (TLR18) mRNA in Selected Species of Food Fishes of India(Indian Veterinary Journal, 2013-07) Raj, K. Jawahar; Uma, A.; Rebecca, G.; Saravanabava, K.; TANUVASThe objective of the study was to identify the existence of TLR18 expression in various food shes of India viz., Channa striata, Pangasius pangasius, Mastacembelus guentheri, Cyprinus carpio, Catla catla, Tilapia mossambicus, Clarias batrachus and Labeo rohita. RT-PCR results showed that the basal expression of TLR18 was present in Pangasius pangasius. The sequence information generated for TLR18 gene was submitted to GenBank (Acc No: JQ627837).ArticleItem Open Access Bluetongue in Migratory Sheep Population(Indian Journal of Small Ruminants, 2005) Balakrishnan, G.; Reddy, Y. Krishnamohan; Saravanabava, K.; Babu, M.; Koteeswaran, A.; TANUVASBluetongue is one of the 15 diseases of OIE list 'A' affecting susceptible domestic and wild ruminants caused by Orbi virus transmitted by Culicoides. The economic losses are mainly attributed to high morbidity, mortality, abortions, stillbirths, foetal abnormalities and loss of milk, meat and fleece. Moreover the presence of this disease in a country puts trade barrier on movement of ruminant animals, their germ plasm and other animal products resulting in animal related international trade losses. The disease prevalence has been reported world wide and widespread in India (Sapre, 1964; Mehrotra, 1991; Janakiraman et al., 1991). In Tamil Nadu, it was first reported in Dindigul region in the year 1982 followed by sporadic incidence in the Southern districts during 1987. This study describes a disease investigation carried out in migratory sheep population suspected for bluetongue in Pudukkottai district of Tamil Nadu during the month of November 2004.ArticleItem Open Access Comparison of Different DNA Extraction Procedures for the Detection of Sheep Pox Virus by PCR(Indian Veterinary Journal, 2009-08) Vadivoo, V.S.; Ramesh, A.; Babu, S. Suresh; Saravanabava, K.; TANUVASScab samples obtained from suspected outbreaks of sheep pox infection were subjected to PCR assay using the Ins-1.1/Ins-1.1' primer pair specific for sheep pox virus. Three known positive clinical samples were subjected to three different procedures of DNA extraction. The DNA template obtained by boiling method showed smearing on the agarose gel electrophoresis. The modified phenol-chloroform method of DNA extraction yielded good quantity of DNA than the commercial DNA extraction kit. The purity of the extracted DNA by both the modified phenol-chloroform method and commercial DNA extraction kit method was found to be almost equal. Thus the modified phenol-chloroform method although time consuming was found to be simple and inexpensive and could be employed for large number of samples. Whereas the expensive commercial DNA extraction kit method could be employed when results are required within a day.OtherItem Open Access Comparison of Infectious Bursal Disease Virus Isolates from Tamil Nadu(TANUVAS, Chennai, 2007-08) Reddy, Y. Krishnamohan; Saravanabava, K.; Thyagarajan, D.; Manohar, B. MuraliArticleItem Open Access CONFIRMATORY DIAGNOSIS OF CONTAGIOUS ECTHYMA BY AMPLIFICATION OF THE GIF / IL-2 GENE BY PCR(TANUVAS, 2008-11) Ramesh, A.; Vadivoo, V.S.; Babu, S. Suresh; Saravanabava, K.; TANUVASArticleItem Open Access Confirmatory Diagnosis of Contagious Ecthyma by PCR and Electron Microscopy(Indian Veterinary Journal, 2009-08) Ramesh, A.; Babu, S. Suresh; Saravanabava, K.; Vadivoo, V.S.; Premalatha, N.; TANUVASOtherItem Open Access Contagious Ecthyma Disease Outbreak and its Confirmation by Electron Microscopic Study(TANUVAS, 2006-06) Ramesh, A.; Saravanabava, K.; Koteeswaran, A.; Mohan, Andrew Chandra; Babu, S. Suresh; Premalatha, N.; Vadivoo, V.S.ArticleItem Open Access Detection of Salmonella from Fish by Polymerase Chain Reaction(Indian Veterinary Journal, 2009-11) Uma, A.; Sudhakar, M.M.; Meena, S.; Saravanabava, K.; Manohar, B. Murali; TANUVASPolymerase Chain Reaction (PCR) assays for the detection of Salmonella from fish and oysters have been reported (Bej et at., 1994). Extraction of DNA is a curcial step, as samples contain PCR inhibitory compounds, which would interfere with the amplification reaction. As the recovery of the pure DNA from samples varies with the extraction method followed, the present study was carried out to know the most suitable DNA extraction method for the PCR assay targeting the himA Gene which codes for DNA binding proteins (Li et al., 1989) of Salmonella isolated from fishes.ArticleItem Restricted Development of a Nested Polmerase Chain Reaction (PCR) Assay to Detect White Spot Syndrome Virus (WSSV) Infecting Shrimp(Indian Veterinary Journal, 2012-06) Uma, A.; Gayathiri, S.; Rebecca, G.; Saravanabava, K.; TANUVASA nested Polymerase chain reaction (PCR) assay was developed for diagnosis of WSSV in Shrimp. The assay was tested to be specific in detecting WSSV. The sensitivity of the first and nested PCR step were 1 pg and 0.01 pg respectively. This assay is useful in detecting WSSV in all life stages of shrimp.ThesisItem Open Access Development Of Experimental Inactivated Vaccine For Bovine Leptospirosis(Tamil Nadu Veterinary and Animal Sciences University, 2009) Balakrishnan, G.; TANUVAS; Roy, Parimal; Saravanabava, K.; Sarathchandra, G.; Parthiban, M.Sero survey against leptospirosis by MAT was conducted on 3605 serum samples collected from cattle and buffaloes suffering from abortion, repeat breeding, jaundice haemorrhagic mastitis and apparently healthy animals from 16 different districts of Tamil Nadu, 12 different districts of Andhra Pradesh and a private farm in Gujarat state. Seropositivity was found to be 40.39 per cent, 50.21 per cent and 34.74 per cent among cattle and 75.66 per cent, 68.64 per cent and 54.14 per cent among buffaloes in Tamil Nadu, Andhra Pradesh and Gujarat respectively. Seropositivity was more in exotic pure breeds (50.00 per cent), followed by indigenous pure breeds (34.38 per cent) and cross breeds (32.74 per cent) cattle. Meanwhile among buffaloes, seropositivity was more in Murrah (58.25 per cent), followed by Pandharpuri (40.91 per cent) and Jaffrabadi (37.50 per cent) respectively. Males were more positive than females. A total of 555 sera samples from different animal conditions which were MAT positive were found for have elevated levels of bilirubin, SGOT and SGPT.Only 115 sera samples from different clinical condition like abortion repeat breeding, jaundice and haemorrhagic mastitis and apparently healthy animals were screened for the presence of Leptospira specific antibody and antigen. Sixty four (55.65 per cent) samples were positive for antibody by MAT whereas 26.09 per cent samples were positive for Leptospira by DFM and 20.87 per cent samples were positive for pathogenic leptospiral DNA by PCR test. Out of 30 sera samples screening for isolation, 9 isolates of australis (5), hebdomadis (3) and ballum (1) were obtained. In sequence analysis no significant variation could be observed with reference strains. Since the serovars australis, ballum, hardjo, hebdomadis and pomona were consistently prevalent, they were selected as seed bacteria for the preparation of Leptospira experimental vaccines. Formalin inactivated two different kinds of adjuvanted pentavalent vaccines were prepared. Vaccine I was adjuvanted with Montanide ISA 206 and vaccine II was adjuvanted with Aluminium hydroxide gel. Both the experimental vaccines were found to be potent and safe. Duration of the immunity for both the vaccines was studied in rabbits under experimental condition and in cattle under field condition. High antibody titre of 6.84 log 2 to 9.64 log 2 with vaccine I (Montanide adjuvanted) and 5.64 to 7.44 log 2 with vaccine II (Aluminium hydroxide adjuvanted) were found at 180 days in rabbits. For cattle, GM – MAT titres of 6.84 log 2 to 7.69 log 2 were observed against vaccine – I at 150 days and such titres were seen with vaccine – II at 120 days. Thus vaccine I was found to be a better vaccine than vaccine II. However both the vaccines showed high immune response of 5.64 log 2 at 6 months of immunization.OtherItem Open Access Development of Veterinary Viral Vaccines and Diagnostics for the Benefit of Farming Community(TANUVAS, Chennai, 2007-08) Saravanabava, K.; Reddy, Y. Krishnamohan; Ramesh, A.; Vadivoo, V.S.; Manohar, B. MuraliArticleItem Open Access Diagnosis of Marek's Disease Virus (MDV) in Commercial Chickens by Slot-Blot Hybridization using PCR based MDV-1 antigen A Gene Probe(2001) Vathsala, M.; Kumanan, K.; Saravanabava, K.; Gunaseelan, L.; TANUVASArticleItem Open Access Differential Expression of Toll-Like Receptor21 (TLR21) in Zebra Fish Danio Rerio Experimentally Exposed to Aeromonas Hydrophila(Indian Veterinary Journal, 2013-03) Uma, A.; Rebecca, G.; Saravanabava, K.; TANUVASThe expression of TLR21 was studied in various organs viz., skin, muscle, gill, liver and intestine by Real time-PCR. Results showed that basal expression of TLR21 was present in the above tissues of zebra fish. A. hydrophila exposure induced the expression of TLR21 significantly in muscle, gill, liver and intestine. It can be concluded that TLR21 recognizes the A. hydrophila, gram-negative bacterial ligand and induction of TLR21 with such bacterial ligand would induce immune response and confer protection against bacterial diseases in fish.ArticleItem Open Access A Differential PCR Assay for Simultaneous Detection of Sheep Pox Virus and Contagious Ecthyma Virus(Indian Veterinary Journal, 2009-12) Vadivoo, V.S.; Ramesh, A.; Babu, S. Suresh; Saravanabava, K.; TANUVASA multiplex PCR based assay (m-PCR) was developed to detect the sheep pox and contagious ecthyma viruses in a single reaction. The two sets of primers used in this PCR assay were Ins-1.1/Ins - 1.1' from the inverted terminal repeats (ITR's) of the sheep pox virus (SPV) and GIF gene/IL-2 inhibition factor of the contagious ecthyma virus (CEV). The m-PCR amplified a 289 bp fragment specific for SPV and a 408 bp fragment of CEV. About 69 scab samples were screened using this assay in which 25 samples were positive for SPV and 44 samples for CEV. The assay proved to be a specific and rapid test for the differential diagnosis of SPV and CEV samples collected from the field outbreak in a single PCR assay.ThesisItem Open Access Efficacy of Different Immunomodulators in Poultry(TANUVAS, 1996) Prasad, B. Arun; TANUVAS; Saravanabava, K.; Albert, A.; Kumanan, K.
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