Detection of Salmonella from Fish by Polymerase Chain Reaction
Loading...
Date
2009-11
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Veterinary Journal
Abstract
Polymerase Chain Reaction (PCR) assays for the detection of Salmonella from fish and oysters have been reported (Bej et at., 1994). Extraction of DNA is a curcial step, as samples contain PCR inhibitory compounds, which would interfere with the amplification reaction. As the recovery of the pure DNA from samples varies with the extraction method followed, the present study was carried out to know the most suitable DNA extraction method for the PCR assay targeting the himA Gene which codes for DNA binding proteins (Li et al., 1989) of Salmonella isolated from fishes.