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Browsing Articles (1.Journal) by Author "Abdul Basith, S"

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    DIAGNOSIS OF BOVINE TUBERCULOSIS IN WILD ANIMALS BY POLYMERASE CHAIN REACTION
    (2007-12) Vathsala, M; Swathika, MC; Jayathangaraj, MG; Gomathinayagam, S; Ramesh, S; Abdul Basith, S; TANUVAS
    Diagnosis of Mycobacterium bovis is important from the public health perspective since control measures, treatment are very significant, particularly in wild animals. To diagnose M. bovis infection in wild animals polymerase chain reaction had been used, using the primer sequence of pnc A 8 and pncA 11 that are specific for M. bovis pnc A gene. A 744 bp PCR product was generated out of 3 samples that consisted of blood, caseated nodule, and lung tissues collected from hyena, bear and lion maintained at Arignar Anna Zoological Park, Chennai, Tamil Nadu and were identified as M. bovis. A positive control of M. bovis DNA procured from Tuberculosis Research Centre (TRC), Chennai, Tamil Nadu was used and a negative control of a healthy bovine blood was used. The present study demonstrates the use of PCR as a sensitive diagnostic gadget for diagnosing bovine tuberculosis (BTB) among wild animals.
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    ArticleItemOpen Access
    Evaluation of PCR and IFAT in the Diagnosis of Canine Ehrlichiosis
    (2009) Asha Rajagopal; Abdul Basith, S; Gomathinayagam, S; Dhinakarraj, G; TANUVAS
    The.results of nested PCR were correlated with that of indirect fluorescent antibody test (IFAT) on 50 matched samples. The results were concordant in 44 out of 50 cases. PCR was concluded to be more suitable than IFAT in the diagnosis of early and subclinical infections of canine ehrlichiosis and served as a reliable indicator for determining the significance of persistent antibody titers.
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    ArticleItemOpen Access
    INCIDENCE OF COCCIDIOSIS IN POULTRY AT NAMAKKAL (TAMIL NADU)
    (1995) Abdul Basith, S; Rajavelu, G; Dorairajan, N; TANUVAS
    The incidence of coccidiosis was studied in chicken brought for necropsy examination at Avian Disease Laboratory, Namakkal.
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    ArticleItemOpen Access
    Prevalence of ectoparasites of chicken reared under different systems of management
    (2008) Rani, N; John, Lalitha; Abdul Basith, S; TANUVAS
    A survey was carried out to determine the incidence of ectoparasites in desi and commercial birds of Tirunelveli and Namakkal district of Tamil Nadu, respectively. Of the 400 desi and 500 commercial birds examined, 298 desi and 270 commercial birds were found to be infested with ectoparasites. Among the ectoparasites encountered viz., fleas Echidnophaga gallinacea (6.7%) only parasitized in desi birds. Three species of lice Menopon gallinae (46.3 and 25.18%), Lipeurus caponis (29.10 and 10%) while Gonioides gigas (9.0%) was seen only on desi birds. Two species of mites Megninia ginglymura (64.8%) and Ornithonysus bursa were seen on commercial and desi birds, respectively.
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    ArticleItemOpen Access
    PREVALENCE OF SUBULURA BRUMPTI IN FOWLS AT NAMAKKAL (TAMIL NADU)
    (1995) Abdul Basith, S; Karunamoorthy, G; TANUVAS
    Subulura brumpti the only ascarid worm with an indirect life cycle, occurs in the caeca of the fowl, turkey, guinea fowl and wild related birds in Africa, North and South America and Asia. In India Bhalerao (1941) recorded S. minetti in the intestine of fowl from Jaipur. From Madras, Ramanujachari and Alwar (1954) recorded S. brumpti in the caecum of fowl. while Alwar and Lalitha (1961) recorded it in the caecum of turkey. Shastri et aI. (1974) recorded S. brumpti from caeca in 8 out of 30 fowls (26.7%) in Maharashtra Ruff (1984) considered S. minetti as a synonym of S brumpti. Perusal of available literature indicates that the incidence of S brumpti in hybrid layers has not been studied in Tamil Nadu. Hence the study was undertaken at Namakkal, Tamil Nadu which is an important poultry belt area in Tamil Nadu.
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    ArticleItemOpen Access
    Serodiagnosis of bovine fasciolosis by Dot-enzyme immuno assay in Chennai, Tamil Nadu
    (2014-01) Latchumikanthan, A; Soundararajan, C; Abdul Basith, S; Dhinarkar Raj, G; TANUVAS
    Excretory/secretory (E/S) antigen of adult Fasciola gigantica was used for diagnosis of fasciolosis in cattle and buffaloes sera from slaughter houses and different places in and around Chennai, Tamil Nadu. A total of 6.4 mg of E/S antigen was obtained from the 60 flukes collected from slaughter houses. Coprological examination was done to detect endoparasitic infection in 321 fecal samples. Dot-enzyme immuno assay (Dot-EIA) was standardized with optimum working dilution of E/S antigen, serum, and conjugate by the checker board titration as 1 in 10 (50 ng/ml), 1 in 20, and 1 in 1,000, respectively. A total of 321 sera samples consisting of 168 cattle and 153 buffaloes were tested by Dot-EIA. A total of 25 cattle (14.88 %) and 38 buffaloes (24.83 %) showed positive results in Dot-EIA. Sensitivity of Dot-EIA for cattle and buffaloes sera was 100 %. Specificity of Dot-EIA for cattle and buffaloes was 95.28 and 92.10 %, respectively in comparison to fecal examination by sedimentation method. Dot-EIA can be easily adapted in the field to detect fasciolosis in cattle and buffaloes.