Serodiagnosis of bovine fasciolosis by Dot-enzyme immuno assay in Chennai, Tamil Nadu
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Date
2014-01
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Abstract
Excretory/secretory (E/S) antigen of adult
Fasciola gigantica was used for diagnosis of fasciolosis in
cattle and buffaloes sera from slaughter houses and different
places in and around Chennai, Tamil Nadu. A total
of 6.4 mg of E/S antigen was obtained from the 60 flukes
collected from slaughter houses. Coprological examination
was done to detect endoparasitic infection in 321 fecal
samples. Dot-enzyme immuno assay (Dot-EIA) was standardized
with optimum working dilution of E/S antigen,
serum, and conjugate by the checker board titration as 1 in
10 (50 ng/ml), 1 in 20, and 1 in 1,000, respectively. A total
of 321 sera samples consisting of 168 cattle and 153 buffaloes
were tested by Dot-EIA. A total of 25 cattle
(14.88 %) and 38 buffaloes (24.83 %) showed positive
results in Dot-EIA. Sensitivity of Dot-EIA for cattle and
buffaloes sera was 100 %. Specificity of Dot-EIA for cattle
and buffaloes was 95.28 and 92.10 %, respectively in
comparison to fecal examination by sedimentation method.
Dot-EIA can be easily adapted in the field to detect fasciolosis
in cattle and buffaloes.
Description
TNV_JPD_2014_38(1)32-35
Keywords
Veterinary Science