Serodiagnosis of bovine fasciolosis by Dot-enzyme immuno assay in Chennai, Tamil Nadu

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dc.contributor.authorLatchumikanthan, A
dc.contributor.authorSoundararajan, C
dc.contributor.authorAbdul Basith, S
dc.contributor.authorDhinarkar Raj, G
dc.contributor.authorTANUVAS
dc.date.accessioned2021-08-27T09:04:40Z
dc.date.available2021-08-27T09:04:40Z
dc.date.issued2014-01
dc.descriptionTNV_JPD_2014_38(1)32-35en_US
dc.description.abstractExcretory/secretory (E/S) antigen of adult Fasciola gigantica was used for diagnosis of fasciolosis in cattle and buffaloes sera from slaughter houses and different places in and around Chennai, Tamil Nadu. A total of 6.4 mg of E/S antigen was obtained from the 60 flukes collected from slaughter houses. Coprological examination was done to detect endoparasitic infection in 321 fecal samples. Dot-enzyme immuno assay (Dot-EIA) was standardized with optimum working dilution of E/S antigen, serum, and conjugate by the checker board titration as 1 in 10 (50 ng/ml), 1 in 20, and 1 in 1,000, respectively. A total of 321 sera samples consisting of 168 cattle and 153 buffaloes were tested by Dot-EIA. A total of 25 cattle (14.88 %) and 38 buffaloes (24.83 %) showed positive results in Dot-EIA. Sensitivity of Dot-EIA for cattle and buffaloes sera was 100 %. Specificity of Dot-EIA for cattle and buffaloes was 95.28 and 92.10 %, respectively in comparison to fecal examination by sedimentation method. Dot-EIA can be easily adapted in the field to detect fasciolosis in cattle and buffaloes.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810174808
dc.keywordsFasciola gigantica Cattle Buffaloes Dot-enzyme immuno assayen_US
dc.language.isoEnglishen_US
dc.pages32-35en_US
dc.relation.ispartofseries;1
dc.subjectVeterinary Scienceen_US
dc.titleSerodiagnosis of bovine fasciolosis by Dot-enzyme immuno assay in Chennai, Tamil Naduen_US
dc.title.alternativeJournal of Parasitic Diseasesen_US
dc.typeArticleen_US
dc.volume38en_US
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