EFFECT OF OVARIAN FOLLICULAR FLUID OF DIFFERENT SPECIES ON IN VITRO MATURATION OF CAPRINE OOCYTES
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Date
2005-11
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY , BIDAR
Abstract
India possesses 136 million goat and stands 2^^ in the world in
goat population, which occupy an important place in Indian farm
economy mainly in meeting the demands for meat
In recent times laboratory production of embryos is drawing keen
attention because of its application in emerging technologies like cloning
and transgenic animal production apart from embryo transfer. With large
number of oocytes, goat serves as a suitable experimental model in
embryo transfer technology, which has been developed for maximum
exploitation of superior genetic material from female.
Since the cost of production of embryos is very high, several efforts
are being made by various research workers to economize the production
by way of replacing the expensive components of culture media with less
expensive ones. In order to reduce the cost of technology; conventionally
used fetal bovine serum, an expensive component and hormones are
being successfully replaced with steer serum, cow serum or follicular
fluid (Gupta et al., 2001).
Follicular fluid is primarily the transudate of plasma that contains
specific constituents such as steroids, glycosaminoglycans and many
other metabolites synthesized by the cells of the follicle wall (Wise, 1987).
Recent studies have claimed the beneficial effects of follicular fluid in
media used in IVM, subsequent fertilization (IVF) and development of pig
(Naito, 1988), sheep (Sun et al., 1994) and cow (Kim et al., 1996)
embryos.
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Keywords
REPRODUCTION, GYNAECOLOGY ,OBSTETRICS