MOLECULAR CHARACTERIZATION, NUTRITIONAL AND ANTINUTRITIONAL PARAMETERS OF PROMISING GENOTYPES OF BLACK GRAM [Vigna mungo (L.) Hepper] 3571
| dc.contributor.advisor | Dr. U. K. Kandoliya | |
| dc.contributor.author | PATEL KALPANABAHEN KHEMABHAI | |
| dc.contributor.other | 2010120068 | |
| dc.date.accessioned | 2023-12-15T17:13:21Z | |
| dc.date.available | 2023-12-15T17:13:21Z | |
| dc.date.issued | 2022-09 | |
| dc.description.abstract | The present investigation on “Molecular Characterization, Nutritional and Antinutritional Parameters of Promising Genotypes of Black Gram [Vigna mungo (L.) Hepper]” was carried out at the Department of Biotechnology, Junagadh Agricultural University, Junagadh with objectives to analyse different 20 selected genotypes of black gram for molecular characterization using various PCR based molecular markers viz. Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSRs) and Simple Sequence Repeat (SSR). Along with, various nutritional and anti-nutritional parameters. The 20 selected genotypes of black gram were selected for the molecular study. Pooled study of molecular marker through RAPD, ISSR and SSR used to confirm the differences and similarity between the black gram genotypes. A total of 11 RAPD primers were amplified with total of 65 bands. There were 62 polymorphic bands out of 65 bands with average of 5.63 bands per primer. The percentage polymorphism obtained for RAPD primers is 94.37 %. The largest fragment of 3200 bp was amplified by CMN- A37 and the smallest fragment of 100 bp was amplified by OPL-03. The primer OPN-14 and CMN-18 amplified one- one unique band. 10 ISSR primers amplified a total of 43 bands. The ISSR marker UBC-811 produced maximum number of 7 bands. The primer UBC-807 amplified two unique band and UBC-825 amplified one unique band. The percentage polymorphism obtained for ISSR primers is 90 %. 12 SSR primers amplified 12 bands. Out 12 bands, 4 bands are monomorphic and 8 bands are polymorphic with 66.67 % polymorphism. All SSR markers produced single band. There was no unique band in SSR analysis. An average polymorphism information content (PIC) value for RAPD primer, ISSR primer and SSR primer were 0.87, 0.91 and 0.00, respectively. Primer index for RAPD, ISSR and SSR were 11.84, 14 and 1, respectively. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810203555 | |
| dc.language.iso | English | |
| dc.pages | 147 | |
| dc.publisher | JAU,JUNAGADH | |
| dc.relation.ispartofseries | 3571 | |
| dc.sub | Agricultural Biotechnology | |
| dc.theme | MOLECULAR CHARACTERIZATION, NUTRITIONAL AND ANTINUTRITIONAL PARAMETERS OF PROMISING GENOTYPES OF BLACK GRAM [Vigna mungo (L.) Hepper] 3571 | |
| dc.these.type | M.Sc | |
| dc.title | MOLECULAR CHARACTERIZATION, NUTRITIONAL AND ANTINUTRITIONAL PARAMETERS OF PROMISING GENOTYPES OF BLACK GRAM [Vigna mungo (L.) Hepper] 3571 | |
| dc.type | Thesis |