MOLECULAR CHARACTERIZATION, NUTRITIONAL AND ANTINUTRITIONAL PARAMETERS OF PROMISING GENOTYPES OF BLACK GRAM [Vigna mungo (L.) Hepper] 3571
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Date
2022-09
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JAU,JUNAGADH
Abstract
The present investigation on “Molecular Characterization, Nutritional and
Antinutritional Parameters of Promising Genotypes of Black Gram [Vigna mungo (L.)
Hepper]” was carried out at the Department of Biotechnology, Junagadh Agricultural
University, Junagadh with objectives to analyse different 20 selected genotypes of black
gram for molecular characterization using various PCR based molecular markers viz.
Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSRs)
and Simple Sequence Repeat (SSR). Along with, various nutritional and anti-nutritional
parameters.
The 20 selected genotypes of black gram were selected for the molecular study.
Pooled study of molecular marker through RAPD, ISSR and SSR used to confirm the
differences and similarity between the black gram genotypes. A total of 11 RAPD primers
were amplified with total of 65 bands. There were 62 polymorphic bands out of 65 bands
with average of 5.63 bands per primer. The percentage polymorphism obtained for RAPD
primers is 94.37 %. The largest fragment of 3200 bp was amplified by CMN- A37 and the
smallest fragment of 100 bp was amplified by OPL-03. The primer OPN-14 and CMN-18
amplified one- one unique band. 10 ISSR primers amplified a total of 43 bands. The ISSR
marker UBC-811 produced maximum number of 7 bands. The primer UBC-807 amplified
two unique band and UBC-825 amplified one unique band. The percentage polymorphism
obtained for ISSR primers is 90 %. 12 SSR primers amplified 12 bands. Out 12 bands, 4
bands are monomorphic and 8 bands are polymorphic with 66.67 % polymorphism. All
SSR markers produced single band. There was no unique band in SSR analysis. An average
polymorphism information content (PIC) value for RAPD primer, ISSR primer and SSR
primer were 0.87, 0.91 and 0.00, respectively. Primer index for RAPD, ISSR and SSR were
11.84, 14 and 1, respectively.