PRASHANTH
dc.contributor.author | PRASHANTH | |
dc.date.accessioned | 2023-12-15T17:40:16Z | |
dc.date.available | 2023-12-15T17:40:16Z | |
dc.date.issued | 2019 | |
dc.description.abstract | A study on bacteriological investigation of canine pyoderma cases was conducted at the Veterinary College, Shivamogga. Exudate/pus/lesion swabs were collected from clinical cases of canine pyoderma (n=126) and subjected to isolation of bacteria, identification of staphylococcal isolates by molecular method and other bacterial isolates by phenotypic methods. The bacteriological processing of the samples resulted in the recovery of 95 staphylococcal isolates and 18 other bacterial isolates. On culture, staphylococci were the most predominantly (n=95, 75.39%) isolated organisms. Amongst staphylococci, S. pseudintermedius (n=82, 86.31%), coagulase positive staphylococci, was the most predominantly organism detected by species-specific nuc PCR. The S. pseudintermedius isolates were further subjected to detection of siet gene by PCR and 84.14% of the isolates were positive. The isolates obtained other than staphylococcal species were E. coli, Pseudomonas species, Klebsiella species, Proteus species. Subsequently in vitro antimicrobial sensitivity testing was carried out for all the isolates. S. pseudintermedius showed highest susceptibility to clindamycin and the highest resistance to enrofloxacin, and the other bacterial agents were found sensitive to co-trimoxazole and ceftriaxone and resistant to amoxicillin/sulbactam and enrofloxacin. Three of the four isolates which were phenotypically methicillin resistant were positive for mecA gene PCR. A study on the occurrence patterns of canine pyoderma revealed higher frequency of cases in male dogs, in the age group of 1-2 years and in Labrador breed. We report the S. pseudintermedius as the most predominant pathogen associated with the canine pyoderma in the study area. The emergence of methicillin resistance in S. pseudintermedius emphasises the need for devise of strategies for its control. | |
dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810203564 | |
dc.pages | 121 | |
dc.publisher | KVAFSU,BIDAR | |
dc.sub | Veterinary Microbiology | |
dc.theme | Veterinary Microbiology | |
dc.these.type | M.V.Sc. | |
dc.title | PRASHANTH | |
dc.type | Thesis |
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