PRASHANTH
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Date
2019
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Journal ISSN
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Publisher
KVAFSU,BIDAR
Abstract
A study on bacteriological investigation of canine pyoderma cases was
conducted at the Veterinary College, Shivamogga. Exudate/pus/lesion swabs were
collected from clinical cases of canine pyoderma (n=126) and subjected to isolation of
bacteria, identification of staphylococcal isolates by molecular method and other
bacterial isolates by phenotypic methods. The bacteriological processing of the
samples resulted in the recovery of 95 staphylococcal isolates and 18 other bacterial
isolates. On culture, staphylococci were the most predominantly (n=95, 75.39%)
isolated organisms. Amongst staphylococci, S. pseudintermedius (n=82, 86.31%),
coagulase positive staphylococci, was the most predominantly organism detected by
species-specific nuc PCR. The S. pseudintermedius isolates were further subjected to
detection of siet gene by PCR and 84.14% of the isolates were positive. The isolates
obtained other than staphylococcal species were E. coli, Pseudomonas species,
Klebsiella species, Proteus species. Subsequently in vitro antimicrobial sensitivity
testing was carried out for all the isolates. S. pseudintermedius showed highest
susceptibility to clindamycin and the highest resistance to enrofloxacin, and the other
bacterial agents were found sensitive to co-trimoxazole and ceftriaxone and resistant
to amoxicillin/sulbactam and enrofloxacin. Three of the four isolates which were
phenotypically methicillin resistant were positive for mecA gene PCR. A study on the
occurrence patterns of canine pyoderma revealed higher frequency of cases in male
dogs, in the age group of 1-2 years and in Labrador breed. We report the S.
pseudintermedius as the most predominant pathogen associated with the canine
pyoderma in the study area. The emergence of methicillin resistance in S.
pseudintermedius emphasises the need for devise of strategies for its control.