STUDIES ON OCCURENCE AND DIAGNOSIS OF CANINE BRUCELLOSIS
| dc.contributor.advisor | A. SAHADEV | |
| dc.contributor.author | BALANGOUDA MALLANAGOUDA PATIL | |
| dc.date.accessioned | 2023-12-29T16:37:39Z | |
| dc.date.available | 2023-12-29T16:37:39Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | A study on occurrence and diagnosis of canine brucellosis was carried out on female dogs presented to the Department of Veterinary Gynaecology Obstetrics clinics, Veterinary College, Bengaluru. A total of 44 female dogs were screened with complaints of abortion (Group-I), history of abortion and mummification (Group-II) and premature birth, still birth and conception failure (Group-III) using ICA (Brucella canis specific) and PCR (genus and species specific). Twenty-six dogs were found positive on bcsp31 PCR (genus-specific) where 9 animals were positive in blood, 9 in tissue (vaginal discharge/washing/aborted tissue) and 8 dogs were found positive on both blood and tissue samples. Out of these, 18 animals belonged to Group-I, 3 to Group-II and 5 to Group-III. Further, the samples were subjected to AMOS and B. canis specific PCR and 16 dogs were found positive for Brucella abortus, 5 animals for B. canis and 5 dogs for both the organisms. In Group-I, 9 dogs were positive for B. abortus, 5 for B. canis and 4 animals for both the species. Group-II had 2 B. abortus positive dogs and 1 dog positive for both the species. Group-III revealed 5 brucella abortus positive dogs. None of the dogs were positive for Brucella ovis and Brucella suis. ICA didn’t show any positive results. Haemetobiochemical studies revealed no significant variation within or between the groups except for mean TLC values between total brucella positive which was significantly higher compared to negative dogs with clinical signs. Current study revealed PCR is highly sensitive for diagnosis of canine brucellosis. Key words: Immunochromatographic assay (ICA), Polymerase chain reaction (PCR), bcsp 31, AMOS, Canine brucellosis. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810205077 | |
| dc.language.iso | English | |
| dc.pages | 115 | |
| dc.publisher | KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR- 585 226 | |
| dc.sub | Veterinary Gynaecology and Obstetrics | |
| dc.theme | Veterinary Gynaecology and Obstetrics | |
| dc.these.type | M.V.Sc. | |
| dc.title | STUDIES ON OCCURENCE AND DIAGNOSIS OF CANINE BRUCELLOSIS | |
| dc.type | Thesis |