STUDIES ON OCCURENCE AND DIAGNOSIS OF CANINE BRUCELLOSIS
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Date
2022
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR- 585 226
Abstract
A study on occurrence and diagnosis of canine brucellosis was carried out on
female dogs presented to the Department of Veterinary Gynaecology Obstetrics clinics,
Veterinary College, Bengaluru. A total of 44 female dogs were screened with complaints
of abortion (Group-I), history of abortion and mummification (Group-II) and premature
birth, still birth and conception failure (Group-III) using ICA (Brucella canis specific) and
PCR (genus and species specific). Twenty-six dogs were found positive on bcsp31 PCR
(genus-specific) where 9 animals were positive in blood, 9 in tissue (vaginal
discharge/washing/aborted tissue) and 8 dogs were found positive on both blood and tissue
samples. Out of these, 18 animals belonged to Group-I, 3 to Group-II and 5 to Group-III.
Further, the samples were subjected to AMOS and B. canis specific PCR and 16 dogs were
found positive for Brucella abortus, 5 animals for B. canis and 5 dogs for both the
organisms. In Group-I, 9 dogs were positive for B. abortus, 5 for B. canis and 4 animals
for both the species. Group-II had 2 B. abortus positive dogs and 1 dog positive for both
the species. Group-III revealed 5 brucella abortus positive dogs. None of the dogs were
positive for Brucella ovis and Brucella suis. ICA didn’t show any positive results.
Haemetobiochemical studies revealed no significant variation within or between the groups
except for mean TLC values between total brucella positive which was significantly higher
compared to negative dogs with clinical signs. Current study revealed PCR is highly
sensitive for diagnosis of canine brucellosis.
Key words: Immunochromatographic assay (ICA), Polymerase chain reaction (PCR),
bcsp 31, AMOS, Canine brucellosis.