ANTIMICROBIAL RESISTANCE AND MOLECULAR EPIDEMIOLOGY OF SALMONELLA AND STAPHYLOCOCCUS SPECIES IN MILK VALUE CHAIN

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dc.contributor.advisorNAGAPPA KARABASANAVAR
dc.contributor.authorSUPRIYA K.
dc.date.accessioned2023-12-18T10:49:31Z
dc.date.available2023-12-18T10:49:31Z
dc.date.issued2021
dc.description.abstractFood safety has become central to the food trade; as milk and dairy products continue to nourish the public, ensuring food safety across the dairy supply chain is of paramount importance. Therefore, a cross sectional study involving 720 samples of the milk value chain was undertaken with the objective of detection of Salmonella and Staphylococcus species as food safety hazards. Prevalence of Salmonella spp was 0.41%; isolates, recovered from milker’s hand, feed and water were found resistant to ampicillin and tetracycline. Staphylococcus species were detected in 4.3% samples of milk supply chain and comprised of coagulase positive (S. aureus 67.7%, S. intermedius 6.4%) and coagulase negative (S. lentus 9.6%, S. sciuri 3.2%, S. xylosus 3.2%, S. schleiferi 3.2%, S. felis 3.2% and S. gallinarum 3.2%) species. Staphylococcus isolates were found resistant to methicillin (32.2%), beta-lactam (41.9%) and macrolide-lincosamide-streptogramin B(3.2%). All the methicillin resistant Staphylococcus isolates carried mecA gene. Questionnaire survey indicated lack and proper information about the antimicrobials use; clean milk producing practices and food safety. The PFGE profiling traced origin of a milk isolate of S. aureus to the soil; however, diverse pulsotypes indicated varied origin of antimicrobial resistant isolates. Findings of this study highlighted need for extensive risk analysis studies involving large samples of milk and dairy products to understand food safety hazards and protect the public health.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810203686
dc.language.isoEnglish
dc.pages150
dc.publisherKARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
dc.subVeterinary Public Health
dc.themeVeterinary Public Health
dc.these.typeM.V.Sc.
dc.titleANTIMICROBIAL RESISTANCE AND MOLECULAR EPIDEMIOLOGY OF SALMONELLA AND STAPHYLOCOCCUS SPECIES IN MILK VALUE CHAIN
dc.typeThesis
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