ANTIMICROBIAL RESISTANCE AND MOLECULAR EPIDEMIOLOGY OF SALMONELLA AND STAPHYLOCOCCUS SPECIES IN MILK VALUE CHAIN
Loading...
Date
2021
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
Food safety has become central to the food trade; as milk and dairy products
continue to nourish the public, ensuring food safety across the dairy supply chain is of
paramount importance. Therefore, a cross sectional study involving 720 samples of
the milk value chain was undertaken with the objective of detection of Salmonella and
Staphylococcus species as food safety hazards. Prevalence of Salmonella spp was
0.41%; isolates, recovered from milker’s hand, feed and water were found resistant to
ampicillin and tetracycline. Staphylococcus species were detected in 4.3% samples of
milk supply chain and comprised of coagulase positive (S. aureus 67.7%, S.
intermedius 6.4%) and coagulase negative (S. lentus 9.6%, S. sciuri 3.2%, S. xylosus
3.2%, S. schleiferi 3.2%, S. felis 3.2% and S. gallinarum 3.2%) species.
Staphylococcus isolates were found resistant to methicillin (32.2%), beta-lactam
(41.9%) and macrolide-lincosamide-streptogramin B(3.2%). All the methicillin
resistant Staphylococcus isolates carried mecA gene. Questionnaire survey indicated
lack and proper information about the antimicrobials use; clean milk producing
practices and food safety. The PFGE profiling traced origin of a milk isolate of S.
aureus to the soil; however, diverse pulsotypes indicated varied origin of
antimicrobial resistant isolates. Findings of this study highlighted need for extensive
risk analysis studies involving large samples of milk and dairy products to understand
food safety hazards and protect the public health.