EFFECTS OF DEXMEDETOMIDINE, MIDAZOLAM AND DEXMEDETOMIDINE-MIDAZOLAM PREMEDICATION IN PROPOFOL INDUCED ANAESTHESIA IN DOGS DURING OVARIOHYSTERECTOMY
| dc.contributor.advisor | Sharma, A.K. | |
| dc.contributor.author | Kumari, Laxmi | |
| dc.date.accessioned | 2017-06-13T10:47:51Z | |
| dc.date.available | 2017-06-13T10:47:51Z | |
| dc.date.issued | 2015 | |
| dc.description | EFFECTS OF DEXMEDETOMIDINE, MIDAZOLAM AND DEXMEDETOMIDINE-MIDAZOLAM PREMEDICATION IN PROPOFOL INDUCED ANAESTHESIA IN DOGS DURING OVARIOHYSTERECTOMY | en_US |
| dc.description.abstract | The present research work was conducted to evaluate the accessibility of dexmedetomidine, midazolam and its combination by propofol infusion on clinicophysiological, anaesthetic, haemodynamics and haemato- biochemical profiles in dogs during ovariohysterectomy procedures. The study was conducted on 20 healthy female dogs, divided randomly into four groups of five animals each and designated as group P, DP, MP and DM. Atropine sulphate was given 20 min prior to administration of propofol in all the groups. In group P, propofol was administered after 20 minutes of atropine. In group DP dexmedetomidine was given @ 20μg/kg b.wt. IM, just 5 minutes after atropine administration thereby propofol “to effect” was administered after 15 minutes of dexmedetomidine administration. Similarly in group MP and DM, midazolam was administered @0.2 mg/kg b.wt and dexmedetomidine and midazolam was taken as 20μg/kg b.wt.+0.2 mg/kg b.wt. in a same syringe and administered to animals after 5 min of atropine and after 15 minutes propofol “ till effect”. Heart rate ( beats/min), respiration rate (/min), rectal temperature (ºF), systolic arterial pressure (mmHg), diastolic arterial pressure (mmHg), mean arterial pressure (mmHg), electrocardiographic study as well as anaesthetic observation such as muscle relaxation, pedal reflex, palpebral reflex, intubation response and salivation were recorded at 0, 10, 20, 30, 45, 60, 90 and 120 minutes of observations. Induction time (min), recovery time (min), sternal-recumbancy time (min), standing recovery time (min), complete recovery time (min) and duration of anaesthesia (min) were also recorded. Haematological parameters like haemoglobin, packed cell volume, total erythrocyte count, total leucocyte count, differential leucocyte count and biochemical parameters like blood urea nitrogen, serum creatinine, serum glucose ALT, AST and total protein were recorded at 0, 30, 60, 120 minutes and at 4 and 24 hours of drug administration. Pain following surgical procedures were analysed by Melbourne pain scale which were started after 30 minutes of recovery from anaesthesia and carried out an interval of 30, 60, 90, 120 minutes and then 1 and 2 hours. In all the groups, there was initially significant increase (P<0.05) in heart rate at 10 and 20 minutes of observation as compared to base line thereafter it decreased throughout the observation. The maximum elevation in heart rate was observed at 10 minute of observation in all the groups. In group DP the significant variation (P<0.05) was observed at 60 minute of observation as compared to base line thereafter it decreased non significantly up to end of observation. There was marked increase in respiration rate in group MP and P after administration of drugs up to 20 minutes of observation. In group DP and DM there was non significant (P>0.05) decrease in respiration rate throughout the end of observation except at 60 minutes in group DP where there was significant variation (P<0.05). The rectal temperatures in all animals were decreased after the administration of drugs. There was significant decrease (P<0.05) in rectal temperature throughout the end of observation in group P. Systolic arterial pressure and diastolic arterial pressure increased upto 20 minutes in all the groups thereafter it decreased upto the end of observation. In group MP there was non significant (P>0.05) variance was observed throughout the end of observation as compared to the base line. A significant elevation of diastolic arterial pressure was observed at 10 minutes in group P and 20 minutes in group DP and DM while significant elevation was observed at 20 minutes in group MP. Muscle relaxation was better in group DM followed by group DP, MP and P at 20 minute interval thereafter profound muscle relaxation was observed upto 45 minute intervals thereby muscle tone improved afterwards. Palpebral reflex completely abolished from 30 to upto 45 minutes intervals in all the groups. Palpebral reflex reappeared firstly in group P (60 min) then in group MP, DM and DP. The pedal reflex score was more in group DM at 20 minutes of interval as compared to other groups. The intubation response was more profound at 30 minutes intervals after administration of propofol. The laryngeal reflex returned first in group MP followed by group P, DM and DP. Induction time was shortest in group DM (1.20±0.20 min) followed by groups DP (2.80±0.49 min), P (3±0.45 min) and MP (4.40±0.40 min). Recovery was longest in group DP (79.00±2.07 min) and shorter in group P (59.40±7.3 min). The animals of group DP attained a longest sternal recumbent (89.60±1.91 min) position than other groups. It was minimal in group P (76.20±6.27 min) followed by groups MP (77.80±1.28 min) and DM (88.80±2.03 min). Standing recovery time was highest in group DP (102.00±2.59 min) followed by groups DM (101.20±2.58 min), MP (84.80±2.40 min) and P (77.40±6.19 min), respectively. The animals of group P (78.60±6.33 min) walked and moved earlier, showing complete recovery of animals than all the other groups while animals of group DM (120.40±2.93 min) had a delayed complete recovery time. Pain score in the animals of all groups were less just after 30 minutes of recovery. In group DP, the values of pain scores showed the decreasing trend from the beginning of observation till end of observation. Animals of group DP exhibited less pain as compared to other groups at all its intervals of observation followed by group DM, P and MP. There was non –significant alteration in the value of heamatogical parameters viz. Hb, PCV, TEC and TLC in all the groups during observation period. While, neutrophilia and lymphocytopenia was the consistent findings in all the groups. Hyperglycaemia was seen in all the groups following administration of drugs. The maximum rise was seen at 1 hour intervals in group DP, MP and DM and at 30 minute intervals in group P. The hypoproteinemia was seen in all the groups and maximum declination was seen at 1 hour interval in group MP and DM and at 4 hour in group P and at 2 hour interval in group DP. There were non significant alterations in the value of ALT, AST, creatinine and BUN in all the groups. It was concluded that dexmedetomidine+midazolam+propofol anaesthesia (DM) was found to be effective and produced satisfactory surgical anaesthesia with significant reduction of propofol as well as produced profound analgesia. Hence it is recommended as better anaesthetic combination over other groups. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810019867 | |
| dc.keywords | EFFECTS OF DEXMEDETOMIDINE, MIDAZOLAM AND DEXMEDETOMIDINE-MIDAZOLAM PREMEDICATION IN PROPOFOL INDUCED ANAESTHESIA IN DOGS DURING OVARIOHYSTERECTOMY | en_US |
| dc.language.iso | en_US | en_US |
| dc.pages | 104 | en_US |
| dc.publisher | Birsa Agricultural University, Kanke, Ranchi, Jharkhand | en_US |
| dc.sub | Veterinary Surgery and Radiology | en_US |
| dc.subject | null | en_US |
| dc.theme | EFFECTS OF DEXMEDETOMIDINE, MIDAZOLAM AND DEXMEDETOMIDINE-MIDAZOLAM PREMEDICATION IN PROPOFOL INDUCED ANAESTHESIA IN DOGS DURING OVARIOHYSTERECTOMY | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | EFFECTS OF DEXMEDETOMIDINE, MIDAZOLAM AND DEXMEDETOMIDINE-MIDAZOLAM PREMEDICATION IN PROPOFOL INDUCED ANAESTHESIA IN DOGS DURING OVARIOHYSTERECTOMY | en_US |
| dc.type | Thesis | en_US |