Isolation and Characterization of Bioactive Compounds from Marine Bacteria

Simple item page
dc.contributor.advisorVenugopal, M.N.
dc.contributor.advisorKrishna Moorthy, M.
dc.contributor.advisorIndrani Karunasagar
dc.contributor.advisorKarunasagar, I
dc.contributor.advisorMaragal, M.M.
dc.contributor.advisorUdupa, K.S.
dc.contributor.authorSuresh, T.
dc.date.accessioned2016-07-04T14:24:30Z
dc.date.available2016-07-04T14:24:30Z
dc.date.issued2009-11-19
dc.descriptionPh.D. Thesisen_US
dc.description.abstractMicroorganisms have been recognized as an important resource for bioactive compounds. Majority of such compounds that are widely used are isolated from terrestrial sources. However, microorganisms isolated from marine sources are known to synthesize metabolites that are unique from the terrestrial counterparts. In recent years marine bacteria are becoming a major source for several novel bioactive compounds. In the present study a total of 1,11, 277 isolates were screened and about 176 bacteria with antimicrobial property were isolated. Among them 64% were identified as Bacillus spp and 36% as Pseudomonas spp. Further screening of these 176 isolates based on zone of inhibition against two indicator bacteria (Staphylococcus aureus as a representative of gram positive bacteria and Vibrio harveyi as representative of gram negative bacteria) led to selection of 28 that showed significant activity. The antimicrobial compounds produced by the strains SM 5 (Bacillus spp) and SK 9 (Pseudomonas spp) inhibited many pathogenic bacteria and the activity was maximum against L. monocytogens, V. fisheri and V.parahemolyticus. Ammonium sulphate fractionation of culture supernatant revealed maximum activity in the 30% fraction. The dialyzed protein fraction extracted from the strain SM 5 also showed promising inhibitory activity against HeLa cell line. As the development of resistance to drugs by pathogenic bacteria is a major concern in the medical science in recent days, isolation of such antimicrobial compounds are very much essential. However the compound isolated in this study needs further study to characterize its unique chemical and antimicrobial properties.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/68411
dc.language.isoenen_US
dc.publisherKarnataka Veterinary, Animal and Fisheries Sciences University, Bidaren_US
dc.subFishery Microbiology
dc.these.typePh.D
dc.titleIsolation and Characterization of Bioactive Compounds from Marine Bacteriaen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
DFK-413- Suresh, T..pdf
Size:
3.24 MB
Format:
Adobe Portable Document Format
Description:
Fisheries: Fishery Microbiology
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.28 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses (Ph.D.)