Isolation and Characterization of Bioactive Compounds from Marine Bacteria
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Date
2009-11-19
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Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar
Abstract
Microorganisms have been recognized as an important resource for bioactive
compounds. Majority of such compounds that are widely used are isolated from terrestrial
sources. However, microorganisms isolated from marine sources are known to synthesize
metabolites that are unique from the terrestrial counterparts. In recent years marine bacteria
are becoming a major source for several novel bioactive compounds. In the present study a
total of 1,11, 277 isolates were screened and about 176 bacteria with antimicrobial
property were isolated. Among them 64% were identified as Bacillus spp and 36% as
Pseudomonas spp. Further screening of these 176 isolates based on zone of inhibition
against two indicator bacteria (Staphylococcus aureus as a representative of gram positive
bacteria and Vibrio harveyi as representative of gram negative bacteria) led to selection of
28 that showed significant activity.
The antimicrobial compounds produced by the strains SM 5 (Bacillus spp) and SK 9
(Pseudomonas spp) inhibited many pathogenic bacteria and the activity was maximum
against L. monocytogens, V. fisheri and V.parahemolyticus. Ammonium sulphate
fractionation of culture supernatant revealed maximum activity in the 30% fraction. The
dialyzed protein fraction extracted from the strain SM 5 also showed promising inhibitory
activity against HeLa cell line. As the development of resistance to drugs by pathogenic
bacteria is a major concern in the medical science in recent days, isolation of such
antimicrobial compounds are very much essential. However the compound isolated in this
study needs further study to characterize its unique chemical and antimicrobial properties.
Description
Ph.D. Thesis