EFFICACY OF ANTITRYPANOSOMAL DRUGS AGAINST TRYPANOSOMA EVANSI BY IN VIVO STUDIES
| dc.contributor.advisor | JAYA NAGAPPA LAKKUNDI | |
| dc.contributor.author | NAGARAJ JALLER | |
| dc.date.accessioned | 2024-01-02T12:26:23Z | |
| dc.date.available | 2024-01-02T12:26:23Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Efficacy of aqueous fresh leaf extract and silver nanoparticles of L. camara (LCAgNPs) along with quinapyramine prosalts were evaluated by in vivo studies in mice model against T. evansi (buffalo isolate) in Karnataka state, India. Ten out of 11 experimental mice groups consisting 8 in each were inoculated with 5x104 T. evansi and one group was maintained as negative control. The infected mice showed parasitaemia on 2nd day post inoculum. One infected group was treated subcutaneously with quinapyramine prosalts at 5 mg/kg b.w. Four infected groups treated with aqueous fresh leaf extract and LCAgNPs at 0.417 and 0.208 g/kg b.w intraperitoneally for three consecutive days. Similarly, four groups treated in combination with quinapyramine prosalts. Two mice from each group sacrificed on 8, 15, 28 and 45th day post treatment for histopathology and PCR analysis. On 5th day 100.0 % mortality observed in positive, aqueous fresh leaf extract and LCAgNPs treated groups and showed congested lungs, hepatomegaly and splenomegaly with major histopathological changes along with organisms in blood vessels of vital organs. Whereas, 12.5 % mortality observed on 30th day in quinapyramine treated group due to relapse and no mortality in other groups with absence of organisms from 2 to 45 days post treatment showed no significant difference between quinapyramine prosalts treated and combination of drug, LCAgNPS and aqueous fresh leaf extract treated groups. PCR analysis of blood of sacrificed mice revealed absence of relapse. The present study concluded post treatment evaluation of quinapyramine prosalts for relapse and further exploration of phytochemicals against trypanosomosis. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810205276 | |
| dc.language.iso | English | |
| dc.pages | 142 | |
| dc.publisher | KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR- 585 401 | |
| dc.sub | Veterinary Parasitology | |
| dc.theme | Veterinary Parasitology | |
| dc.these.type | M.V.Sc. | |
| dc.title | EFFICACY OF ANTITRYPANOSOMAL DRUGS AGAINST TRYPANOSOMA EVANSI BY IN VIVO STUDIES | |
| dc.type | Thesis |