EFFICACY OF ANTITRYPANOSOMAL DRUGS AGAINST TRYPANOSOMA EVANSI BY IN VIVO STUDIES
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Date
2022
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR- 585 401
Abstract
Efficacy of aqueous fresh leaf extract and silver nanoparticles of L. camara
(LCAgNPs) along with quinapyramine prosalts were evaluated by in vivo studies in mice
model against T. evansi (buffalo isolate) in Karnataka state, India. Ten out of 11
experimental mice groups consisting 8 in each were inoculated with 5x104 T. evansi and
one group was maintained as negative control. The infected mice showed parasitaemia on
2nd day post inoculum. One infected group was treated subcutaneously with
quinapyramine prosalts at 5 mg/kg b.w. Four infected groups treated with aqueous fresh
leaf extract and LCAgNPs at 0.417 and 0.208 g/kg b.w intraperitoneally for three
consecutive days. Similarly, four groups treated in combination with quinapyramine
prosalts. Two mice from each group sacrificed on 8, 15, 28 and 45th day post treatment
for histopathology and PCR analysis. On 5th day 100.0 % mortality observed in positive,
aqueous fresh leaf extract and LCAgNPs treated groups and showed congested lungs,
hepatomegaly and splenomegaly with major histopathological changes along with
organisms in blood vessels of vital organs. Whereas, 12.5 % mortality observed on 30th
day in quinapyramine treated group due to relapse and no mortality in other groups with
absence of organisms from 2 to 45 days post treatment showed no significant difference
between quinapyramine prosalts treated and combination of drug, LCAgNPS and
aqueous fresh leaf extract treated groups. PCR analysis of blood of sacrificed mice
revealed absence of relapse. The present study concluded post treatment evaluation of
quinapyramine prosalts for relapse and further exploration of phytochemicals against
trypanosomosis.