Characterization of Excretory and Secretory Larval Antigen of Toxocara canis by Western Blotting
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Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
Excellent Publishers
Abstract
Toxocara canis is a dog helminth which causes visceral larva migrans (VLM) in humans.
The identification of specific antigens of T. canis is important in order to develop better
diagnostic techniques. The excretory-secretory larval antigens of T. canis (ESLA) were
prepared by in vitro culturing of T. canis larvae in RPMI 1640 medium. These antigens
were separated using sodium dodecyl sulphate-poly acrylamide gel electrophoresis (SDSPAGE)
which revealed 9 protein bands at a molecular weight of 17, 18, 22, 24, 26, 28, 30,
32 and 120 kDa. The immuno reactivity of excretory-secretory larval antigens of T. canis
was checked by Western blotting using hyper immune serum raised in rabbits against
ESLA antigen which showed six immuno reactive bands at a molecular weight of 17, 18,
22, 24, 30 and 32 kDa. These antigens merit further evaluation as candidate for use in
diagnosis of toxocariasis in humans and adult dogs.
Description
TNV_IJCMAS_2019_8(5)804-811
Keywords
Veterinary Science