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Authors: Kavitha, KT
Sreekumar, C
Latha, BR
Mangala Gowri, A
Nagarajan, B
Title: Characterization of Excretory and Secretory Larval Antigen of Toxocara canis by Western Blotting
Other Titles: International Journal of Current Microbiology and Applied Sciences
Publisher: Excellent Publishers
Language: en
Type: Article
Pages: 804-811
Volume: 8
Series/Report no.: ;5
Agrotags: Veterinary Science
Keywords: Toxocara canis, Excretory-secretory larval antigens, Western blotting
Abstract: Toxocara canis is a dog helminth which causes visceral larva migrans (VLM) in humans. The identification of specific antigens of T. canis is important in order to develop better diagnostic techniques. The excretory-secretory larval antigens of T. canis (ESLA) were prepared by in vitro culturing of T. canis larvae in RPMI 1640 medium. These antigens were separated using sodium dodecyl sulphate-poly acrylamide gel electrophoresis (SDSPAGE) which revealed 9 protein bands at a molecular weight of 17, 18, 22, 24, 26, 28, 30, 32 and 120 kDa. The immuno reactivity of excretory-secretory larval antigens of T. canis was checked by Western blotting using hyper immune serum raised in rabbits against ESLA antigen which showed six immuno reactive bands at a molecular weight of 17, 18, 22, 24, 30 and 32 kDa. These antigens merit further evaluation as candidate for use in diagnosis of toxocariasis in humans and adult dogs.
Description: TNV_IJCMAS_2019_8(5)804-811
Issue Date: 2019
Appears in Collections:Articles (1.Journal)

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