Characterization of Excretory and Secretory Larval Antigen of Toxocara canis by Western Blotting

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dc.contributor.authorKavitha, KT
dc.contributor.authorSreekumar, C
dc.contributor.authorLatha, BR
dc.contributor.authorMangala Gowri, A
dc.contributor.authorNagarajan, B
dc.date.accessioned2019-11-04T11:58:28Z
dc.date.available2019-11-04T11:58:28Z
dc.date.issued2019
dc.descriptionTNV_IJCMAS_2019_8(5)804-811en_US
dc.description.abstractToxocara canis is a dog helminth which causes visceral larva migrans (VLM) in humans. The identification of specific antigens of T. canis is important in order to develop better diagnostic techniques. The excretory-secretory larval antigens of T. canis (ESLA) were prepared by in vitro culturing of T. canis larvae in RPMI 1640 medium. These antigens were separated using sodium dodecyl sulphate-poly acrylamide gel electrophoresis (SDSPAGE) which revealed 9 protein bands at a molecular weight of 17, 18, 22, 24, 26, 28, 30, 32 and 120 kDa. The immuno reactivity of excretory-secretory larval antigens of T. canis was checked by Western blotting using hyper immune serum raised in rabbits against ESLA antigen which showed six immuno reactive bands at a molecular weight of 17, 18, 22, 24, 30 and 32 kDa. These antigens merit further evaluation as candidate for use in diagnosis of toxocariasis in humans and adult dogs.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810134163
dc.keywordsToxocara canis, Excretory-secretory larval antigens, Western blottingen_US
dc.language.isoenen_US
dc.pages804-811en_US
dc.publisherExcellent Publishersen_US
dc.relation.ispartofseries;5
dc.subjectVeterinary Scienceen_US
dc.titleCharacterization of Excretory and Secretory Larval Antigen of Toxocara canis by Western Blottingen_US
dc.title.alternativeInternational Journal of Current Microbiology and Applied Sciencesen_US
dc.typeArticleen_US
dc.volume8en_US
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