STUDIES ON BACTERIAL FLORA IN BOVINE AND BUBALINE SEMEN OF BREEDING BULLS IN KARNATAKA
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Date
2022
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR-585401
Abstract
The semen samples were collected from three semen collection centres located in
and around Bangalore. Eight HF bulls and five bubaline bulls from Farm-A, twelve HF
bulls from Farm-B, eight HF bulls and eleven bubaline bulls from Farm-C, were selected
for the study. Three ejaculates from each bull at fortnightly intervals were collected and
subjected for the estimation of total bacterial load in the neat, extender and frozen semen
to evaluate the level of contamination occurring during handling, processing and storage
of semen. The bacterial load of the semen was found to be within the prescribed guidelines
of BIS suggestive of the hygienic measures followed during semen collection, processing
and preservation. The isolates were subjected to the genus level typing using cultural,
staining characteristics and molecular methods. The isolates isolated in the present study
were Staphylococcus spp., Bacillus spp., Klebsiella spp., Corynebacterium spp., Serratia
spp. and Proteus spp., etc. The antibiogram of Staphylococcus isolates were highly
sensitive to Imipenem, followed by Piperacillin Tazobactam and Ofloxacin. Bacillus,
Klebsiella, Corynebacterium and Proteus isolates were highly sensitive to Imipenem,
Piperacillin Tazobactam. Serratia isolates were highly sensitive to Piperacillin
Tazobactam. The study revealed that the conventional antibiotics used in the semen stations
were sufficient to reduce the bacterial load in the semen along with strict hygienic measures
during the collection and processing of semen. But it was found that the conventionally
used antibiotics like Penicillin G and Streptomycin were resistant to many bacterial isolates
obtained in the present study.