SYNTHESIS AND EVALUATION OF PLANT BASED SILVER NANOPARTICLES ON MUSCID FLIES
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Date
2022
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR- 585 401
Abstract
An insecticidal efficacy of AgNPs synthesized from aqueous leaf extracts of E.
tereticornis (Et-AgNPs) and P. pinnata (Pp-AgNPs) was evaluated on muscid flies by in
vitro assays at different concentrations. The highest mortality of 70.0 per cent was observed
against the adult flies of S. calcitrans and M. domestica in Et and Pp-AgNPs, at 20 mg
concentration after 24 hours exposure. The LC25(1.226, 1.073, 1.013 and 0.420, 0.203,
0.458), LC50(9.158, 9.218, 8.736 and 5.705, 6.482, 2.798) and LC90(467.65, 546.86, 523.49
and 809.5, 467.26, 86.99) values were observed for Et and Pp-AgNPs against all the adult
flies, respectively. Et and Pp-AgNPs showed 75.0 and 85.0, 100.0 and 90.0 and 80.0 and
85.0 per cent of larval mortality for M. domestica, S. calcitrans and H. irritans irritans at
20 mg concentration after 48 hours exposure, respectively. The LC25(5.053, 0.0873, 1.5280
and 1.546, 1.166, 2.683), LC50(12.873, 2.294, 5.239 and 6.222, 3.508, 5.702) and
LC90(76.086, 14.375, 54.485 and 87.715, 28.455, 23.871) values were indicated for all the
three muscid larvae, respectively. The pupicidal assay indicated the highest per cent
inhibition against S. calcitrans (45.0 and 40.0%) followed by M. domestica (30.0 and
30.0%) and H. irritans irritans (30.0 and 30.0%) at 8 mg concentration after 24 hours
exposure for Et and Pp-AgNPs, respectively. The LC25(1.714, 0.412, 4.20 and 1.908, 3.00
and 4.757), LC50(6.270, 1.921, 30.386 and 28.229, 19.955 and 17.599) and LC90(73.675,
35.801, 62.32 and 4.719, 730.52 and 211.367) values were observed for Et and Pp-AgNPs
against all the three pupae, respectively. Probit analysis revealed the significant effect with
different concentration of AgNPs on adult, larvae and pupal mortality of muscid flies. The
study indicated Et-AgNPs and Pp-AgNPs