STUDIES ON PATHOLOGY OF FCA [FREUND’S COMPLETE ADJUVANT] INDUCED RHEUMATOID ARTHRITIS MODEL IN RATS AND AMELIORATIVE EFFECTS OF GARLIC DERIVED LECTIN
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Date
2022
Authors
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Journal ISSN
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR-585401
Abstract
The present study was undertaken to evaluate the anti-inflammatory and antiarthritic
efficacy of a garlic derived lectin in rats with Freund's complete adjuvant
induced rheumatoid arthritis. Eighty two rats of uniform age were randomly divided
into six groups consisting of six rats each in acute phase and Group I of subacute phase
and eight rats each in Group II to VI of subacute phase and treated as follows.
Rheumatoid arthritis was induced in rats of groups II to VI by injecting intradermally,
Freund's complete adjuvant (FCA) @ 0.1ml/100g body weight into the left hind paw
on Day 0 and Day 8 (Booster dose for subacute phase). Treatment protocols were
initiated on day 0 following FCA injection and continued until the end of the study.
The groups were normal control (Group I, NC), rheumatoid arthritis control (Group II,
RAC), rheumatoid arthritis rats treated with garlic derived lectin @ 5mg/kg bw by IP
route (Group III, GL5), rheumatoid arthritis rats treated with garlic derived lectin @
10mg/kg bw by IP route (Group IV, GL10), rheumatoid arthritis rats treated with garlic
derived lectin @ 20mg/kg bw by IP route (Group V, GL20) and rheumatoid arthritis
rats treated with Methotrexate @ 50μg/kg bw/week by PO route (Group VI, MTX).
Compared to rheumatoid arthritis control, there was a significant improvement
in evaluatory parameters such as body weight, paw volume, paw thickness, decrease in
arthritic score and increase in mobility score, remarkably improved haematological,
sero-biochemical, arthritic markers (CRP & RF), antioxidant, radiographic changes in
treatment groups. Histopathologically, the treatment groups showed substantial
improvement in lesions like pannus formation, rhabdomyositis and periosteitis in the
acute phase study and considerable improvement in cartilage erosion and degradation
in the subacute phase study when compared to the rheumatoid arthritis control group.
Thus, the current study concludes that a mid and higher dose of garlic derived lectin
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@10mg/kg and 20mg/kg, respectively, has significant anti-inflammatory and
antiarthritic activity.
Key words: Rheumatoid Arthritis, Freund’s complete adjuvant, Garlic derived lectin,
Methotrexate