ISOLATION AND MOLECULAR CHARACTERIZATION OF E. coli FROM DIARRHOEIC CASES OF PIGS
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Date
2023
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
E. coli is a common and commensal in the pig’s intestinal tract. However, some strains cause illness in both humans and animals and survive in the intestinal tract which leads to diarrhea and antibiotic resistance. Therefore, it is necessary to identify E. coli which causes diarrhea in pigs. A quick diagnosis of pathogenic E. coli is now achievable because of the development of PCR, which makes it possible to find pathogenic E. coli in bacterial isolates. The goal of the current research was to study the isolation, identification, biochemical characterization, antibiogram profile and molecular characterization of E. coli isolates by PCR. In the present study a total of 102 faecal samples were collected from suspected diarrhoeal cases of swine from different pig farms of Karnataka during the period July 2022 to January 2023 were analysed. The collected samples were subjected for cultural isolation, morphological identification and biochemical characterization. Based on cultural, morphological and biochemical identification out of 102 samples, 79 samples were found positive for E. coli which accounted for 77.45 per cent. The positive samples identified by biochemical tests were subjected to PCR for confirmation of E. coli isolates. According to the PCR method, out of the 102 samples that were processed, 66 samples were found to be positive for E. coli which accounts for 64.70 per cent. The antibiotic sensitivity test was performed in accordance with the standard guidelines. E. coli isolates found highly sensitive to Gentamicin (HLG), Ceftriaxone (CTR) and Chloramphenicol (C). Intermediate sensitivity towards Streptomycin and Ampicillin (A/S). Highly resistance towards Cefepime (CPM) and Amoxycillin (AMC). While resistant towards Enrofloxacin (EX) and Tetracycline (TE).