ISOLATION AND CHARACTERIZATION OF VIBRIO MIMICUS AND VIBRIO (GRIMONTIA) HOLLISAE FROM SEAFOOD
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Date
2020
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
healthy diet. Major health risks are involved in the consumption of raw or undercooked
seafood that may be naturally contaminatedby foodborne pathogens present in the marine
environment. Group of Vibrios are associated with live seafood, asthey are the indigenous
microflora of the marine environment. Seafood contaminated withpathogenic Vibrios not only
play an important role in the transmission of diseases but also act as areservoir in the marine
realms. As the foodborne infections of Vibrio spp. are common in seafood, it is necessary to
survey thepresence of toxigenic and non-toxigenic Vibrios from various seafood and their
environment.
In the present study, 50 different seafood samples were collected from Mangaluru
(Dakshina Kannada District), Mulki, Udupi and Kundapura (Udupi District), Bhatkal, Ankola,
and Karwar (Uttara Kannada District). A total of 365 isolates were suspected as Vibrios from
the collected samples that were plated on different isolation media. Out of 365 suspected
isolates, 3 isolates of V. mimicus and 1 isolate of V. hollisae were confirmed phenotypically by
performing battery of biochemical tests. These isolates were further confirmed by performing
Polymearse Chain Reaction (PCR) using specific primers, in which AraC gene was targeted to
V. mimicus (488 bp) and gyrB gene to V. hollisae (363 bp).Phenotypically confirmed
V. mimicus showed negative result as they failed to amplify at specific basepair and V. hollisae
showed positive result by amplifying at its specific base pair. For further confirmation, 16S
rRNA sequence analysis was done by out sourcing for the genotypically confirmed
V .hollisae. The BLAST sequence analysis, revealed that V.hollisae strain matches with
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V. alginolyticus, but 16S rRNA is closely related to Grimontia hollisae sequences present in
NCBI gene database. Haemolyitc assay was also performed for the phenotypically confirmed
V. mimicus and V. hollisae to test their virulence.V. mimicus showed no haemolysis on blood
agar plate while V. hollisae showed a clear zone of haemolysis (β-hemolysis). This present
study reveals that the samples collected from the study area did not show the presence of
V.mimicus.