STANDARDIZATION OF IMMUNOPEROXIDASE TESTS FOR THE DIAGNOSIS OF RABIES
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Date
1984
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Birsa Agricultural University
Abstract
The present study was conducted to standardize tests which are comparable in specificity to Fluorescent antibody and Mouse inoculation tests for the diagnosis of rabies. A total of 95 specimens, consisting of 53 infected and 42 normal brain tissue were tested. The infected specimens included 11 CVS-infected mice brains, eight specimens from field cases and 34 brains from the mice infected with the field material. The 42 normal brain originate from mouse.
Immunoperoxidase tests vis., Direct IPT, Indirect IPT and Peroxidase-Anti-Peroxidase (PAP) alongwith Indirect Fluorescent Antibody Test (PAT) were standardized for the specific diagnosis of rabies. Simultaneously, mouse inoculation, Direct FAT and Seller's staining were carried out.
While each of these 4 tests gave 100.0 percent specific reaction, the sensitivity differed. The sensitivity of Direct IPT was 95.3 percent, compared to 98.0 percent of Indirect IPT and 100.0 percent of each of PAP and Indirect FAT. The immunoperoxidase tests perform well on the formalin preserved and paraffin embedded tissues and it was easy to recognize the reaction on the histopathological sections. Histopathological sections were also stained by Seller's and Haematoxylere and Eosin stain to demonstrate Negri bodies.
The study was extended to see whether the acetone fixed smears stored at room temperature could be stained specifically for the viral antigen. The viral antigen could be detected only up to 4 days.
Description
STANDARDIZATION OF IMMUNOPEROXIDASE TESTS FOR THE DIAGNOSIS OF RABIES