GENETIC DIVERSITY IN SYNTHETIC CHICKEN BREEDS RAJASRI AND VANARAJA AND THEIR DIVERGENCE FROM ASEEL USING MITOCHONDRIAL COI GENE
Loading...
Files
Date
2022-04
Journal Title
Journal ISSN
Volume Title
Publisher
SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
Rural backyard poultry plays an important role in improving food and nutritional
security and reducing livelihood insecurity for the poorest households. Backyard
farming's low productivity has led many researchers to develop synthetic colored chicken
breeds as an alternative. Rajasri and Vanaraja are two such synthetic chicken breeds
developed with a motive of improving productivity of backyard poultry besides
maintaining the consumer preference for colored chicken and brown shelled eggs. In the
present study, it was aimed to characterize the mitochondrial COI region of the Rajasri,
Vanaraja breeds from Andhra Pradesh and study their genetic diversity with respect to
Aseel breed and explore the possibility of identifying the breeds using this COI gene.
Genomic DNA was isolated using phenol-chloroform method from the already
collected blood samples of six unrelated individuals belonging to each of the three genetic
groups. A 651 bp of mitochondrial DNA encompassing COI region was amplified using
primers available from the published literature. The amplicons were sequenced using
Sanger’s method. The sequences were aligned to other Indian native chicken breeds, Red
jungle fowls from India, China, Japan, Myanmar, layer birds from Germany, Plymouth
rock from USA, leghorns from Japan, Canada. A total of 70 sequences were obtained
from the NCBI database. The DNA polymorphism and divergence among the three
genetic groups, genetic differentiation estimates were measured using DNASP v6
software. DNA barcode signatures among the three genetic groups, phylogenetic analysis
was performed using MEGA X software. The results showed that the DNA
polymorphism is relatively low in the three genetic groups. Among the three populations
Rajasri is highly differentiated from both Aseel and Vanaraja. The analysis revealed the
potentiality of COI gene as a barcode in identifying Rajasri birds through a (m.7488 G>A)
substitution. The phylogenetic analysis revealed monophyletic origin of the three genetic
groups represented as a single clade and sub clustering of Rajasri birds within the first
clade
Description
THESES