GENETIC DIVERSITY IN SYNTHETIC CHICKEN BREEDS RAJASRI AND VANARAJA AND THEIR DIVERGENCE FROM ASEEL USING MITOCHONDRIAL COI GENE
| dc.contributor.advisor | MURALIDHAR, M(MAJOR) | |
| dc.contributor.advisor | SUDHAKAR, K | |
| dc.contributor.advisor | IQBAL HYDER | |
| dc.contributor.author | BALA SAI SUBHADRA DEVI, MADDIMSETTI | |
| dc.date.accessioned | 2023-02-10T07:00:57Z | |
| dc.date.available | 2023-02-10T07:00:57Z | |
| dc.date.issued | 2022-04 | |
| dc.description | THESES | en_US |
| dc.description.abstract | Rural backyard poultry plays an important role in improving food and nutritional security and reducing livelihood insecurity for the poorest households. Backyard farming's low productivity has led many researchers to develop synthetic colored chicken breeds as an alternative. Rajasri and Vanaraja are two such synthetic chicken breeds developed with a motive of improving productivity of backyard poultry besides maintaining the consumer preference for colored chicken and brown shelled eggs. In the present study, it was aimed to characterize the mitochondrial COI region of the Rajasri, Vanaraja breeds from Andhra Pradesh and study their genetic diversity with respect to Aseel breed and explore the possibility of identifying the breeds using this COI gene. Genomic DNA was isolated using phenol-chloroform method from the already collected blood samples of six unrelated individuals belonging to each of the three genetic groups. A 651 bp of mitochondrial DNA encompassing COI region was amplified using primers available from the published literature. The amplicons were sequenced using Sanger’s method. The sequences were aligned to other Indian native chicken breeds, Red jungle fowls from India, China, Japan, Myanmar, layer birds from Germany, Plymouth rock from USA, leghorns from Japan, Canada. A total of 70 sequences were obtained from the NCBI database. The DNA polymorphism and divergence among the three genetic groups, genetic differentiation estimates were measured using DNASP v6 software. DNA barcode signatures among the three genetic groups, phylogenetic analysis was performed using MEGA X software. The results showed that the DNA polymorphism is relatively low in the three genetic groups. Among the three populations Rajasri is highly differentiated from both Aseel and Vanaraja. The analysis revealed the potentiality of COI gene as a barcode in identifying Rajasri birds through a (m.7488 G>A) substitution. The phylogenetic analysis revealed monophyletic origin of the three genetic groups represented as a single clade and sub clustering of Rajasri birds within the first clade | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810193572 | |
| dc.keywords | CHICKEN BREEDS; RAJASRI; VANARAJA; DIVERGENCE; ASEEL; GENETIC DIVERSITY; SYNTHETIC; MITOCHONDRIAL COI GENE | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 76 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA | en_US |
| dc.sub | Animal Genetics and Breeding | en_US |
| dc.theme | GENETIC DIVERSITY IN SYNTHETIC CHICKEN BREEDS RAJASRI AND VANARAJA AND THEIR DIVERGENCE FROM ASEEL USING MITOCHONDRIAL COI GENE | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | GENETIC DIVERSITY IN SYNTHETIC CHICKEN BREEDS RAJASRI AND VANARAJA AND THEIR DIVERGENCE FROM ASEEL USING MITOCHONDRIAL COI GENE | en_US |
| dc.type | Thesis | en_US |