Expression of putative chitinase of Rhipicephalus sanguineus sensu lato in Rosetta (DE3)pLysS cells
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Date
2021-12
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Abstract
with cell density, aerobic conditions
and the toxic effect of metabolite produced post induction. In the current study, the
ideal expression conditions needed to obtain optimum yield of a putative chitinase from
Rhipicephalus sanguineus sensu lato using recombinant Rosetta (DE3)pLysS cells was
evaluated. The expressed proteins were visualised in SDS-PAGE and confirmed by
western blotting. Double selection of colonies expressing the recombinant protein was
carried in the study. The study showed that induction at an OD600 range of 0.6-0.8 and
aerobic conditions with the induction volume being one-fifth the flask volume favoured
the expression of recombinant protein. Post-induction incubation periods of 1- 16hr did
not influence protein production among the induced cultures.
Description
TNV_JVP_2021_35(2)120-125
Keywords
Veterinary Science