Expression of putative chitinase of Rhipicephalus sanguineus sensu lato in Rosetta (DE3)pLysS cells

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dc.contributor.authorV. Sagar, Sharath
dc.contributor.authorRavi Latha, Bhaskaran
dc.contributor.authorSangaran, A, et al.,
dc.contributor.authorTANUVAS
dc.date.accessioned2022-05-10T11:45:39Z
dc.date.available2022-05-10T11:45:39Z
dc.date.issued2021-12
dc.descriptionTNV_JVP_2021_35(2)120-125en_US
dc.description.abstractwith cell density, aerobic conditions and the toxic effect of metabolite produced post induction. In the current study, the ideal expression conditions needed to obtain optimum yield of a putative chitinase from Rhipicephalus sanguineus sensu lato using recombinant Rosetta (DE3)pLysS cells was evaluated. The expressed proteins were visualised in SDS-PAGE and confirmed by western blotting. Double selection of colonies expressing the recombinant protein was carried in the study. The study showed that induction at an OD600 range of 0.6-0.8 and aerobic conditions with the induction volume being one-fifth the flask volume favoured the expression of recombinant protein. Post-induction incubation periods of 1- 16hr did not influence protein production among the induced cultures.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810184555
dc.keywordsPET32(a)(+), Rosetta (DE3) pLysS, SDS-PAGE, Western Blotting, Chitinase, Rhipicephalus sanguineus.en_US
dc.language.isoEnglishen_US
dc.pages120-125en_US
dc.relation.ispartofseries;2
dc.subjectVeterinary Scienceen_US
dc.titleExpression of putative chitinase of Rhipicephalus sanguineus sensu lato in Rosetta (DE3)pLysS cellsen_US
dc.title.alternativeJournal of Veterinary Parasitologyen_US
dc.typeArticleen_US
dc.volume35en_US
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