Evaluation of recombinant outer membrane protein based vaccine against Salmonella Typhimurium in birds
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Date
2013
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Abstract
Food-borne diseases caused by Salmonella enterica from poultry sources represent an important public
health problem and no reliable control by vaccination has proved effective despite research. The aim of
the present study was to evaluate the use of recombinant OmpC protein for immunization of birds to
elucidate its protection against virulent Salmonella Typhimurium. The recombinant OmpC protein was
prepared after cloning and expressing ompC gene and was characterized by SDS-PAGE and Western blot
analyses. The protein preparations were tested as vaccine candidate in layer birds by comparing the
immune response, protection and organ clearance against crude lysate and control. The biologically
functional recombinant 43 kDa truncated OmpC protein proved to be a good immunogen which induced
a significantly high humoral immune response than control. At the same time, it primed a stable cellmediated
immune response. A protective index (based on faecal shedding of organism) of rOmpC
based preparations ranged between 50 and 75% as observed for 3 weeks after challenge. Therefore, the
protein preparations conferred satisfactory protection against challenge infections with virulent strains
of S. Typhimurium as evidenced by limited faecal shedding and minimal detection of Salmonella from
edible tissues and eggs. These findings suggest the possibility to explore the use of S. enterica OMP
protein for the production of novel vaccine.
Description
TNV_Biological_30_2013_1-7
Keywords
Veterinary Science, Veterinary Public Health and Epidemiology