Evaluation of recombinant outer membrane protein based vaccine against Salmonella Typhimurium in birds

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dc.contributor.authorPrejit
dc.contributor.authorAgarwal, Rajesh Kumar
dc.contributor.authorPorteen, Kannan
dc.contributor.authorDubal, Zunjar B.
dc.contributor.authorAsha, Karthikeyan
dc.contributor.authorShweta, Singh
dc.contributor.authorRipan, Biswas
dc.contributor.authorTANUVAS
dc.date.accessioned2022-03-22T09:22:17Z
dc.date.available2022-03-22T09:22:17Z
dc.date.issued2013
dc.descriptionTNV_Biological_30_2013_1-7en_US
dc.description.abstractFood-borne diseases caused by Salmonella enterica from poultry sources represent an important public health problem and no reliable control by vaccination has proved effective despite research. The aim of the present study was to evaluate the use of recombinant OmpC protein for immunization of birds to elucidate its protection against virulent Salmonella Typhimurium. The recombinant OmpC protein was prepared after cloning and expressing ompC gene and was characterized by SDS-PAGE and Western blot analyses. The protein preparations were tested as vaccine candidate in layer birds by comparing the immune response, protection and organ clearance against crude lysate and control. The biologically functional recombinant 43 kDa truncated OmpC protein proved to be a good immunogen which induced a significantly high humoral immune response than control. At the same time, it primed a stable cellmediated immune response. A protective index (based on faecal shedding of organism) of rOmpC based preparations ranged between 50 and 75% as observed for 3 weeks after challenge. Therefore, the protein preparations conferred satisfactory protection against challenge infections with virulent strains of S. Typhimurium as evidenced by limited faecal shedding and minimal detection of Salmonella from edible tissues and eggs. These findings suggest the possibility to explore the use of S. enterica OMP protein for the production of novel vaccine.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810183709
dc.keywordsVeterinary Science, Veterinary Public Health and Epidemiology, Chicken Immune response OMP ompC gene Protective index Salmonella Vaccineen_US
dc.language.isoEnglishen_US
dc.pages1-7en_US
dc.subjectVeterinary Scienceen_US
dc.subjectVeterinary Public Health and Epidemiologyen_US
dc.titleEvaluation of recombinant outer membrane protein based vaccine against Salmonella Typhimurium in birdsen_US
dc.typeArticleen_US
dc.volume30en_US
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