Clinicopathological studies of PPR (Peste des petits ruminants) in sheep and goats along with molecular characterization of the virus in Odisha
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Date
2020
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Department of Veterinary Pathology, OUAT, Bhubaneswar
Abstract
There were total fourteen suspected outbreaks of PPR occurring in almost eight
districts of Odisha comprising of 361 small ruminants. There was 49.65% mortality in goats
and 36% mortality among sheep. There was highest occurrence of PPR among the age groups
6 -12 months (46.13%), females (80.49%), Ganjam breeds (45.51%) and in animals with poor
body conditions (54.48%). There was a significant increase in Hb, TEC, PCV and TLC both
in affected goats and sheep with nutrophillia and lymphopaenia. There was a significant
increase in creatinine, urea, BUN, AST, ALT, K+
and Na+concentrations in PPR affected small ruminants. Prominent patho-anatomical changes were soiled hind quarters and tails,
erosive & ulcerative lesions on oral mucosa, crusts on nose, matting of eyelids, pseudo
membrane like deposits on tongue, frothy exudates as well as haemorrhages in trachea along
with antero-ventral consolidation of lungs. There was desquamation of lingual papillae along
with infiltration of inflammatory cells in papillary area of tongue, sinusoidal dilatation with
congestion and hemorrhage associated with disruption of hepatic cords, presence of
intracytoplasmic inclusions in hepatocytes, villous atrophy, depletion of spleenic pulp and
congestion, congestion of inter alveolar septa with infiltration of macrophages, plasma cells
with syncytia formation, I/n eosinophilic inclusions in alveolar macrophage of lungs as well
as I/c eosinoiphillic inclusions in alveolar epithelial cells were important findings in affected
animals in our study. The gene sequence information was submitted to the NCBI database
with GenBank accession no. MG748708, MG748709, MG748710, MG748711. The current
isolates were clustered to Asian lineage IV with a close relation to earlier reported Indian
strains such as Delhi (KX033350.1) and Tamil Nadu (KT860065.1 and KT860064.1). The
result revealed that the current isolate is 98.8 %-100% identical with previously reported
Indian isolates. It has > 95% identity with lineage IV and 91% -94 % identities with lineage I,
II and III. The overall divergence calculated for the current study was less than 10%. Lineage
IV differs primarily at 13 amino acid positions compared to other lineages. There were four
unique mutations (E297D, W333G, G461R, and E512G).
Description
Th-6071