Simulated physiological oocyte maturation (SPOM) improves developmental competence of in vitro produced goat embryos
Loading...
Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The effect of simulated physiological oocyte maturation on the developmental competence, reactive
oxygen species production and apoptosis rate of in vitro produced goat embryos were studied in the
present experiment. Oocytes and spermatozoa were recovered from ovaries and epididymis, respectively,
procured from a local small animal abattoir. The oocytes aspirated from the ovaries were allocated into
two groups, control (subjected to routine in vitro maturation, fertilization and culture) and simulated
physiological oocyte maturation (SPOM) group (subjected to prematuration, followed by routine in vitro
maturation, fertilization and culture). The SPOM group showed a significantly (p < 0.05) higher maturation
and blastocyst rates (90.60 ± 0.46% and 29.09 ± 2.59%, respectively) as compared to the control
group (85.29 ± 0.98% and 24.09 ± 1.08%). The intensity of reactive oxygen species of the embryos in the
control group (14.98 ± 0.83 pixels/embryo) was significantly (p < 0.05) higher than the SPOM group
(9.60 ± 0.76 pixels/embryo). The apoptosis rate was also significantly (p < 0.05) higher in the embryos of
the control group (9.18 ± 1.07%) as compared to the SPOM group (5.71 ± 0.90%). In conclusion, the
simulated physiological oocyte maturation system significantly increases the developmental competence
of the oocytes and decreases the intensity of reactive oxygen species and embryonic apoptosis in abattoir
derived goat embryos.
Description
TNV_THER_2021_172(1)
Keywords
Veterinary Science